Protein Info for MPMX20_03300 in Enterobacter sp. TBS_079

Annotation: Purine nucleoside phosphorylase 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01699: xanthosine phosphorylase
  • amino acids 24 to 271 (248 residues), 497.7 bits, E=6.2e-154
• TIGR01697: inosine/guanosine/xanthosine phosphorylase family
  • amino acids 24 to 269 (246 residues), 346.9 bits, E=5.7e-108
• PF01048: PNP_UDP_1
  • amino acids 25 to 271 (247 residues), 153.4 bits, E=3.4e-49

Best Hits

Swiss-Prot: 87% identical to XAPA_ECOLI: Purine nucleoside phosphorylase 2 (xapA) from Escherichia coli (strain K12)

KEGG orthology group: K03815, xanthosine phosphorylase [EC: 2.4.2.-] (inferred from 89% identity to ent:Ent638_2934)

MetaCyc: 87% identical to xanthosine phosphorylase (Escherichia coli K-12 substr. MG1655)
Purine-nucleoside phosphorylase. [EC: 2.4.2.1, 2.4.2.15]; 2.4.2.1 [EC: 2.4.2.1, 2.4.2.15]; 2.4.2.1 [EC: 2.4.2.1, 2.4.2.15]; 2.4.2.- [EC: 2.4.2.1, 2.4.2.15]

Predicted SEED Role

"Xanthosine phosphorylase (EC 2.4.2.1)" in subsystem Xanthosine utilization (xap region) (EC 2.4.2.1)

MetaCyc Pathways

• superpathway of purine nucleotide salvage (13/14 steps found)
• superpathway of purine deoxyribonucleosides degradation (7/7 steps found)
• purine ribonucleosides degradation (6/6 steps found)
• adenine and adenosine salvage III (4/4 steps found)
• purine deoxyribonucleosides degradation I (4/4 steps found)
• purine nucleotides degradation II (aerobic) (9/11 steps found)
• ureide biosynthesis (6/7 steps found)
• adenine and adenosine salvage V (3/3 steps found)
• purine deoxyribonucleosides degradation II (3/3 steps found)
• superpathway of guanine and guanosine salvage (3/3 steps found)
• adenine and adenosine salvage I (2/2 steps found)
• guanine and guanosine salvage I (2/2 steps found)
• xanthine and xanthosine salvage (2/2 steps found)
• guanosine nucleotides degradation III (3/4 steps found)
• inosine 5'-phosphate degradation (3/4 steps found)
• adenosine nucleotides degradation II (3/5 steps found)
• nucleoside and nucleotide degradation (archaea) (4/10 steps found)
• fluoroacetate and fluorothreonine biosynthesis (1/6 steps found)
• nucleoside and nucleotide degradation (halobacteria) (1/6 steps found)
• arsenic detoxification (mammals) (8/17 steps found)
• salinosporamide A biosynthesis (3/15 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• Nicotinate and nicotinamide metabolism
• Purine metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.-, 2.4.2.1

Use Curated BLAST to search for 2.4.2.- or 2.4.2.1 or 2.4.2.15

Protein Sequence (274 amino acids)

>MPMX20_03300 Purine nucleoside phosphorylase 2 (Enterobacter sp. TBS_079)
MTLSNNPWFSADIIRAAKPDFTPRVAFILGSGLGALAEQIVDAVVLSYEKLPGFPVSTVH
GHAGELVLGHLAGVPVACMKGRGHFYEGRGIAVMTDAIRTFKLLGCELLFSTNAAGSLRP
EVEPGSLVALSDHINTLPGTPMVGLNDDRFGDRFFSLANAYDADCRALLQTVASEKGFPL
HEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVVPEVISARHCGLKVVAVSAITNLAEG
LSDVKLSHEQTLAAAELSRQNFIDLICGFLRQLA