Protein Info for MPMX20_03172 in Enterobacter sp. TBS_079

Annotation: Alpha-ketoglutarate-dependent dioxygenase AlkB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 216 transmembrane" amino acids 142 to 158 (17 residues), see Phobius details PF13532: 2OG-FeII_Oxy_2" amino acids 18 to 210 (193 residues), 206 bits, see alignment E=3.5e-65

Best Hits

Swiss-Prot: 77% identical to ALKB_ECOLI: Alpha-ketoglutarate-dependent dioxygenase AlkB (alkB) from Escherichia coli (strain K12)

KEGG orthology group: K03919, alkylated DNA repair protein [EC: 1.14.11.-] (inferred from 89% identity to enc:ECL_03516)

MetaCyc: 77% identical to DNA oxidative demethylase (Escherichia coli K-12 substr. MG1655)
RXN-21233 [EC: 1.14.11.54]; 1.14.11.54 [EC: 1.14.11.54]; RXN-18737 [EC: 1.14.11.54, 1.14.11.33]; 1.14.11.33 [EC: 1.14.11.54, 1.14.11.33]; 1.14.11.33 [EC: 1.14.11.54, 1.14.11.33]; 1.14.11.33 [EC: 1.14.11.54, 1.14.11.33]; 1.14.11.33 [EC: 1.14.11.54, 1.14.11.33]; 1.14.11.- [EC: 1.14.11.54, 1.14.11.33]; RXN0-7275 [EC: 1.14.11.54, 1.14.11.33]

Predicted SEED Role

"Alkylated DNA repair protein AlkB" in subsystem DNA repair, bacterial

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.14.11.- or 1.14.11.33 or 1.14.11.54

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (216 amino acids)

>MPMX20_03172 Alpha-ketoglutarate-dependent dioxygenase AlkB (Enterobacter sp. TBS_079)
MLDLFADAEPWQEPLAPGAVILRRFALSRAAALLAGIDEVAAVSPFRHMVTPGGYTMSVA
MTNCGELGWATNAQGYVYAPGDPVTGQPWPPMPDVFRALCHDAAVEAGYADFRPDACLIN
RYAVGAKLSLHQDKDEPDLRAPIVSVSLGLPAVFQFGGLRRNDPLKRLMLEHGDVVVWGR
ESRLFYHGIQPLKPGVHPQTGEFRLNLTFRQASRGQ