Protein Info for MPMX20_01463 in Enterobacter sp. TBS_079

Annotation: Trans-4-hydroxy-L-proline dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01774: glycyl radical enzyme, PFL2/glycerol dehydratase family
  • amino acids 8 to 810 (803 residues), 1365.7 bits, E=0
• PF02901: PFL-like
  • amino acids 10 to 671 (662 residues), 692.5 bits, E=6.9e-212
• PF01228: Gly_radical
  • amino acids 688 to 792 (105 residues), 109.5 bits, E=1.2e-35

Best Hits

Swiss-Prot: 94% identical to PFLF_ECOLI: Putative formate acetyltransferase 3 (ybiW) from Escherichia coli (strain K12)

KEGG orthology group: K00656, formate C-acetyltransferase [EC: 2.3.1.54] (inferred from 97% identity to enc:ECL_02896)

Predicted SEED Role

"Pyruvate formate-lyase (EC 2.3.1.54)" in subsystem Butanol Biosynthesis or Fermentations: Mixed acid (EC 2.3.1.54)

MetaCyc Pathways

• mixed acid fermentation (16/16 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (18/19 steps found)
• superpathway of N-acetylneuraminate degradation (19/22 steps found)
• superpathway of L-threonine metabolism (16/18 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (9/9 steps found)
• L-threonine degradation I (6/6 steps found)
• pyruvate fermentation to acetate IV (3/3 steps found)
• pyruvate fermentation to ethanol I (3/3 steps found)
• reductive monocarboxylic acid cycle (2/2 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (7/13 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Propanoate metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.54

Use Curated BLAST to search for 2.3.1.54

Protein Sequence (810 amino acids)

>MPMX20_01463 Trans-4-hydroxy-L-proline dehydratase (Enterobacter sp. TBS_079)
MTTQNLNTLSERIKAHKTALVHIVKPPVCTERALHYTEMYQQHMDKPIPVRRALALAHHL
AQRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDLADRPGAGFAVSEENKRVL
HEICPWWRGQTVQDRCYGMFTDEQKGLLETGIIKAEGNMTSGDAHLAVNFPLLLEKGLDG
LRDKVAERRSRINLTVLDDLHGEQFLKAIDIVLEAVSLHIERFAALAREMAATESRESRR
DELLAMAENCDVIAHQPPTTFWQALQLCYFIQLILQIESNGHSVSFARMDQYLYPYYRRD
VELNQSLDREHAIELLHSCWLKLLEVNKIRSGSHSKASAGSPLYQNVTIGGQTLINGEPM
DAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFLDACVQVIRCGFGMPAFNNDEIVI
PEFIKLGVEKEDAYDYAAIGCIETAVGGKWGYRCTGMSFINFARVMLAALEGGRDATSGK
VFLPQEKALSAGNFDNFEEVMAAWDTQIRYYTRKSIEIEYVVDTMLEENVPDILCSALVD
DCIERAKSIKQGGAKYDWVSGLQVGIANLGNSLAAVKKLVFEQGTIGQQQLAAALADDFD
GLTHEQLRQRLINGAPKYGNDDDSVDMLLTRAYETYIAELKQYHNPRYGRGPIGGNYYAG
TSSISANVPFGAATMATPDGRKAHTPLAEGASPASGTDHLGPTAVIGSVGKLPTEAILGG
VLLNQKLNPSTLENDSDRQKLMVLLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDL
VVRVAGYSAFFTALSPDAQDDIIARTEHTL