Protein Info for MPMX20_01406 in Enterobacter sp. TBS_079

Annotation: ATP-dependent dethiobiotin synthetase BioD 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13500: AAA_26
  • amino acids 9 to 215 (207 residues), 141.9 bits, E=2.3e-45
• TIGR00347: dethiobiotin synthase
  • amino acids 11 to 181 (171 residues), 214.1 bits, E=6.7e-68
• PF01656: CbiA
  • amino acids 11 to 221 (211 residues), 60.9 bits, E=1.3e-20

Best Hits

Swiss-Prot: 77% identical to BIOD_ESCVU: ATP-dependent dethiobiotin synthetase BioD (bioD) from Escherichia vulneris

KEGG orthology group: K01935, dethiobiotin synthetase [EC: 6.3.3.3] (inferred from 90% identity to enc:ECL_02958)

MetaCyc: 75% identical to dethiobiotin synthetase (Escherichia coli K-12 substr. MG1655)
Dethiobiotin synthase. [EC: 6.3.3.3]

Predicted SEED Role

"Dethiobiotin synthetase (EC 6.3.3.3)" in subsystem Biotin biosynthesis (EC 6.3.3.3)

MetaCyc Pathways

• biotin biosynthesis I (15/15 steps found)
• biotin biosynthesis from 8-amino-7-oxononanoate I (4/4 steps found)
• biotin biosynthesis from 8-amino-7-oxononanoate II (3/4 steps found)
• biotin biosynthesis II (4/6 steps found)
• biotin biosynthesis from 8-amino-7-oxononanoate III (3/5 steps found)

KEGG Metabolic Maps

• Biotin metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.3.3

Use Curated BLAST to search for 6.3.3.3

Protein Sequence (238 amino acids)

>MPMX20_01406 ATP-dependent dethiobiotin synthetase BioD 1 (Enterobacter sp. TBS_079)
MGLLNVTERYFVTGTDTEVGKTVASSALLQAARLQGKTTAGYKPVASGSEMTAHGLRNTD
ALALQRNSTLELAYSAVNPYTFAEPTSPHIISADEGRPIDFAVLSAGLRALERQADWVLV
EGAGGWFTPLSADQTFADWVQIEQLPVILVVGVKLGCINHAMLTAKAVQQAGLRLAGWIA
NDVVAPGRRHQEYLATLKRVLPAPFLGEIPWLADTAGCAETGHYLDLSVLPPATSSGR