Protein Info for MPMX20_01248 in Enterobacter sp. TBS_079

Annotation: Enterobactin synthase component E

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02275: (2,3-dihydroxybenzoyl)adenylate synthase
  • amino acids 4 to 527 (524 residues), 864.2 bits, E=1.4e-264
• PF00501: AMP-binding
  • amino acids 35 to 395 (361 residues), 221.5 bits, E=1.6e-69
• PF13193: AMP-binding_C
  • amino acids 446 to 520 (75 residues), 43 bits, E=7.3e-15

Best Hits

Swiss-Prot: 79% identical to ENTE_ECOLI: Enterobactin synthase component E (entE) from Escherichia coli (strain K12)

KEGG orthology group: K02363, enterobactin 2,3-dihydroxybenzoate-AMP ligase / S-dihydroxybenzoyltransferase [EC: 2.3.1.- 2.7.7.58] (inferred from 92% identity to enc:ECL_03107)

MetaCyc: 79% identical to 2,3-dihydroxybenzoate-AMP ligase (Escherichia coli K-12 substr. MG1655)
2,3-dihydroxybenzoate--serine ligase. [EC: 6.2.1.71, 6.3.2.14]; 2.7.7.- [EC: 6.2.1.71, 6.3.2.14]

Predicted SEED Role

"2,3-dihydroxybenzoate-AMP ligase (EC 2.7.7.58) [enterobactin] siderophore" in subsystem Siderophore Enterobactin (EC 2.7.7.58)

MetaCyc Pathways

• superpathway of chorismate metabolism (54/59 steps found)
• enterobactin biosynthesis (7/11 steps found)
• vibriobactin biosynthesis (4/9 steps found)
• bacillibactin biosynthesis (6/12 steps found)
• shinorine biosynthesis (3/8 steps found)
• vanchrobactin biosynthesis (3/8 steps found)
• acinetobactin biosynthesis (3/9 steps found)
• anguibactin biosynthesis (2/8 steps found)
• myxochelin A and B biosynthesis (2/9 steps found)
• colibactin biosynthesis (7/38 steps found)

KEGG Metabolic Maps

• 1- and 2-Methylnaphthalene degradation
• Alkaloid biosynthesis I
• Alkaloid biosynthesis II
• Anthocyanin biosynthesis
• Benzoate degradation via CoA ligation
• Biosynthesis of siderophore group nonribosomal peptides
• Biosynthesis of terpenoids and steroids
• Biosynthesis of type II polyketide backbone
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Carotenoid biosynthesis - General
• Diterpenoid biosynthesis
• Ether lipid metabolism
• Ethylbenzene degradation
• Fatty acid biosynthesis
• Glycerophospholipid metabolism
• Glycosphingolipid biosynthesis - ganglio series
• Histidine metabolism
• Limonene and pinene degradation
• Lipopolysaccharide biosynthesis
• Lysine degradation
• Phenylalanine metabolism
• Tyrosine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.-

Use Curated BLAST to search for 2.3.1.- or 2.7.7.58 or 6.2.1.71 or 6.3.2.14

Protein Sequence (536 amino acids)

>MPMX20_01248 Enterobactin synthase component E (Enterobacter sp. TBS_079)
MTLPFTRWPEDFARRYREKGYWQDLPLTHILTDQANNDAVAIVDGDRRITYREFNQSVNN
LASRLQALGLHRGETALVQLGNVCEFYMTFFALLQVGVAPVNALFSHQRSELNAYAAQIK
PALLIADREHALFAGDDFLNTFVDEHRSVRVVLLRGDKGEHALDAAIAHPAENFIPNPTP
PDEVAFFQLSGGSTGTPKLIPRTHNDYDYSIRRSNAICGITADTRYLNALPAAHNYAMSS
PGSLGVFTAGGCVVLANDPSATICFPLIEKHQITVTSLVPPAVSLWLQAIADGAGNAPLS
SLTLLQVGGARLSATLAARIPAEIGCQLQQVFGMAEGLVNYTALDDTPERIINTQGRPMC
PDDEVWVADEHGNPLPRGEVGRLMTRGPYTFRGYFNSPEHNASAFDADGFYCSGDLIAID
EQGYITVQGREKDQINRGGEKIAAEEIENLLLRHDAVIHAALVSMEDSLLGEKSCAYLVV
KEPLRAVEVRRFLREQGVAEFKLPDRVESVDALPLTPVGKVDKKQLRLWLAERARG