Protein Info for MPMX20_00897 in Enterobacter sp. TBS_079

Annotation: Hydroxyacylglutathione hydrolase GloB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 252 TIGR03413: hydroxyacylglutathione hydrolase" amino acids 4 to 251 (248 residues), 339.4 bits, see alignment E=5.7e-106 PF00753: Lactamase_B" amino acids 15 to 165 (151 residues), 86.9 bits, see alignment E=2.7e-28 PF12706: Lactamase_B_2" amino acids 25 to 89 (65 residues), 29.8 bits, see alignment E=6.7e-11 PF16123: HAGH_C" amino acids 166 to 251 (86 residues), 80.8 bits, see alignment E=1.2e-26

Best Hits

Swiss-Prot: 84% identical to GLO2_ENT38: Hydroxyacylglutathione hydrolase (gloB) from Enterobacter sp. (strain 638)

KEGG orthology group: K01069, hydroxyacylglutathione hydrolase [EC: 3.1.2.6] (inferred from 90% identity to enc:ECL_01011)

MetaCyc: 75% identical to hydroxyacylglutathione hydrolase GloB (Escherichia coli K-12 substr. MG1655)
Hydroxyacylglutathione hydrolase. [EC: 3.1.2.6]

Predicted SEED Role

"Hydroxyacylglutathione hydrolase (EC 3.1.2.6)" in subsystem Glutathione: Non-redox reactions or Methylglyoxal Metabolism (EC 3.1.2.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (252 amino acids)

>MPMX20_00897 Hydroxyacylglutathione hydrolase GloB (Enterobacter sp. TBS_079)
MNLISIPAFQDNYIWVLVDDERRCVIVDPGESAPVPDAIKENGWQPEAILLTHHHNDHTG
GVADLRAQFPHLVVYGPAETQDKGTTQVVEEGENILIREWEFSVFATPGHTLGHLCFYSK
PYLFCGDTLFSGGCGRLFEGTPAQMYQSLQKISALPDDTLICCAHEYTLGNMKFAASILP
EDRAIQDYYHKVKELRAKNQKTLPVILKNERKTNLFLRTDDIDLIDEINKETNLSQPEQR
FAWLRSKKDNFR