Protein Info for MPMX20_00784 in Enterobacter sp. TBS_079
Annotation: UDP-N-acetylmuramate--L-alanine ligase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 94% identical to MURC_CITK8: UDP-N-acetylmuramate--L-alanine ligase (murC) from Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)
KEGG orthology group: K01924, UDP-N-acetylmuramate--alanine ligase [EC: 6.3.2.8] (inferred from 99% identity to enc:ECL_00888)MetaCyc: 94% identical to UDP-N-acetylmuramate--L-alanine ligase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylmuramate--L-alanine ligase. [EC: 6.3.2.8]
Predicted SEED Role
"UDP-N-acetylmuramate--alanine ligase (EC 6.3.2.8)" in subsystem Peptidoglycan Biosynthesis (EC 6.3.2.8)
MetaCyc Pathways
- peptidoglycan biosynthesis I (meso-diaminopimelate containing) (12/12 steps found)
- UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
- UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
- UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
- peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
- peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
- peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
- peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 6.3.2.8
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (491 amino acids)
>MPMX20_00784 UDP-N-acetylmuramate--L-alanine ligase (Enterobacter sp. TBS_079) MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGYQISGSDLAPNPVTQQL ASLGATIYFNHRPENVRDASVVVVSSAISSDNPEIVAAHEARIPVIRRAEMLAELMRFRH GIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAGVHARLGHSRYLIAEADESDA SFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFLHNLPFYGRAVMCVDDPVIRELL PRVGRQITTYGFSEDADVRVEDYKQLGAQGHFTLARQDKELLHVTLNAPGRHNALNAAAA VAVATEEGIDDEAILRALESFQGTGRRFDFLGEFPLEAVNGKPGTAMLVDDYGHHPTEVD ATIKAARAGWPEKNLVMIFQPHRFTRTRDLYDDFASVLSQVDTLLMLDVYAAGETPIPGA DSRSLCRTIRGRGKVDPILVSDPAQVAEILAPVLTGNDLILIQGAGNIGKIARTLAEIKL KPQTQEDEHHG