Protein Info for MPMX20_00781 in Enterobacter sp. TBS_079
Annotation: UDP-N-acetylmuramoylalanine--D-glutamate ligase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 90% identical to MURD_SALPA: UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) from Salmonella paratyphi A (strain ATCC 9150 / SARB42)
KEGG orthology group: K01925, UDP-N-acetylmuramoylalanine--D-glutamate ligase [EC: 6.3.2.9] (inferred from 97% identity to enc:ECL_00885)MetaCyc: 89% identical to UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase. [EC: 6.3.2.9]
Predicted SEED Role
"UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC 6.3.2.9)" in subsystem Peptidoglycan Biosynthesis (EC 6.3.2.9)
MetaCyc Pathways
- peptidoglycan biosynthesis I (meso-diaminopimelate containing) (12/12 steps found)
- UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
- UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
- peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
- peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
- peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
- peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 6.3.2.9
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (438 amino acids)
>MPMX20_00781 UDP-N-acetylmuramoylalanine--D-glutamate ligase (Enterobacter sp. TBS_079) MADYQGKKVVIIGLGLTGLSCVDFFLARGVTPRVMDTRVSPPGLDKLPEQVERHLGGLND DWLLAADLIVASPGMALAHPSLSAAADAGVEIVGDIELFCREAQAPVIAITGSNGKSTVT TLVGEMAKAAGMNVGVGGNIGLPALMLLDKGCELYVLELSSFQLETTSSLRAAAATILNV TEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALTMPVRGADDRCISFGITMGDYHL NRQLGETWLRVKGEKVLNVKEMKLTGQHNYTNALAALALADAVGLPRSSSLQALTTFTGL AHRFQLALEHNGVRWINDSKATNVGSTEAALNGLHVEGTLHLLLGGDGKSADFSTLKPYL AGNNIRLYCFGRDGSELAELRPDIAEQTETMEQAMRLIAPRVKPGDMVLLSPACASLDQF KNFEQRGDLFTRLAKELG