Protein Info for MPMX20_00058 in Enterobacter sp. TBS_079

Annotation: Multidrug resistance protein MexA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 373 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details PF00529: CusB_dom_1" amino acids 31 to 349 (319 residues), 43.7 bits, see alignment E=4.3e-15 TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 33 to 363 (331 residues), 260.3 bits, see alignment E=1.1e-81 PF16576: HlyD_D23" amino acids 58 to 281 (224 residues), 51.5 bits, see alignment E=1.7e-17 PF13533: Biotin_lipoyl_2" amino acids 58 to 104 (47 residues), 29.8 bits, see alignment 8e-11 PF13437: HlyD_3" amino acids 167 to 278 (112 residues), 32.9 bits, see alignment E=1.9e-11

Best Hits

Swiss-Prot: 49% identical to ACRA_ECO57: Multidrug efflux pump subunit AcrA (acrA) from Escherichia coli O157:H7

KEGG orthology group: K03585, membrane fusion protein (inferred from 93% identity to enc:ECL_00055)

MetaCyc: 49% identical to multidrug efflux pump membrane fusion lipoprotein AcrA (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-1551; TRANS-RXN-1552; TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-356; TRANS-RXN-357; TRANS-RXN-359; TRANS-RXN-360; TRANS-RXN0-592

Predicted SEED Role

"Probable RND efflux membrane fusion protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (373 amino acids)

>MPMX20_00058 Multidrug resistance protein MexA (Enterobacter sp. TBS_079)
MKAITTSIAALFLLTGCDNAQTSAPQHPLPQVGIVTLLSQPVSVVSELTGRTTAAMSAEV
RPQVGGIIQKRLFTEGDTVQAGQALYQIDPSSYRAAYDEAAAALKQAQALVQADCQKAQR
YTQLVKDDGVSRQDAEDAKSTCTQDKASVESKKAAQESARINLNWTTVTAPIAGRIGISS
VTPGALVTAQQETALATIRGLDNMYVDLTRSSADLLRLRKQTLASNSETLSVSLILEDGS
TYQEKGRLALTEVAVDESTGSVTLRAVFPNPQHQLLPGMFVRARVDEGVMSEAILAPQQG
ITRDAKGNATALVVGAGNKVEQRQLETGDTYGDKWLVLSGLKAGDKLIVEGTDKVTAGQQ
VNAEEMKTAGGNA