Protein Info for MMP_RS08025 in Methanococcus maripaludis S2

Annotation: coenzyme-B sulfoethylthiotransferase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 553 PF02745: MCR_alpha_N" amino acids 5 to 272 (268 residues), 482.6 bits, see alignment E=3e-149 TIGR03256: methyl-coenzyme M reductase, alpha subunit" amino acids 6 to 552 (547 residues), 1007.6 bits, see alignment E=8.4e-308 PF02249: MCR_alpha" amino acids 319 to 444 (126 residues), 221.6 bits, see alignment E=3.6e-70

Best Hits

Swiss-Prot: 94% identical to MCRA_METVA: Methyl-coenzyme M reductase subunit alpha (mcrA) from Methanococcus vannielii

KEGG orthology group: K00399, methyl-coenzyme M reductase alpha subunit [EC: 2.8.4.1] (inferred from 100% identity to mmp:MMP1559)

MetaCyc: 77% identical to alpha subunit (Methanothermobacter thermautotrophicus)
Coenzyme-B sulfoethylthiotransferase. [EC: 2.8.4.1]

Predicted SEED Role

"Methyl coenzyme M reductase alpha subunit (EC 2.8.4.1)" in subsystem Methanogenesis (EC 2.8.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.8.4.1

Use Curated BLAST to search for 2.8.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6LWZ5 at UniProt or InterPro

Protein Sequence (553 amino acids)

>MMP_RS08025 coenzyme-B sulfoethylthiotransferase subunit alpha (Methanococcus maripaludis S2)
MEAEKRLFLKALKEKFEEDPKEKYTKFYTFGGWEQSARKREFVEANEKIVSEKRQGIPLY
NPDIGVPLGQRKLMPYKLSNTDDYCEGDDLHFLNNAAIQQLWDDIRRTVIVGMDTAHSVL
EKRLGVEVTPETINEYMHTINHSLPGGAVVQEHMVEVHPSLAWDCYARIFTGDDELADEL
DSRFLIDINKLFPEEQAETLKAAIGKKTYQVSRVPSLVGRVCDGGTISRWSAMQIGMSFI
TAYKLCAGEAATADFSYASKHADVIQMGNALPGRRARGPNEPGGIRFGILSDVVQTTRVS
EDPVEQSLEVVATGAALYDQIWLGAYMSGGVGFTQYATASYTDDILDDFSYYALDYVEKK
YGRMGTKATMDVVEDVAGEVTLYALEQYDDYPALLEDHFGGSQRAAVAAAASGIGVCMAT
GNSNAGVNGWYLSQILHKEYHSRLGFYGYDLQDQCGASNSLAIRNDEAAPLELRGPNYPN
YAMNVGHQGEYAGIAQAAHSARGDAFALSPLVKVAFADPMLVFDFSKPRKEMARGALREF
EAAGERDVILPAK