Protein Info for MMP_RS07610 in Methanococcus maripaludis S2

Annotation: homoaconitase large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 418 TIGR02086: 3-isopropylmalate dehydratase, large subunit" amino acids 1 to 417 (417 residues), 609.4 bits, see alignment E=2.9e-187 TIGR01343: homoaconitate hydratase family protein" amino acids 2 to 417 (416 residues), 624.5 bits, see alignment E=6.8e-192 PF00330: Aconitase" amino acids 5 to 288 (284 residues), 203.7 bits, see alignment E=3e-64 amino acids 288 to 413 (126 residues), 127.5 bits, see alignment E=3.8e-41

Best Hits

Swiss-Prot: 68% identical to HACA_METJA: Methanogen homoaconitase large subunit (hacA) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

KEGG orthology group: K01705, homoaconitate hydratase [EC: 4.2.1.36] (inferred from 100% identity to mmp:MMP1480)

MetaCyc: 68% identical to homoaconitase alpha subunit (Methanocaldococcus jannaschii)
Methanogen homoaconitase. [EC: 4.2.1.114, 4.2.1.36]; 4.2.1.114 [EC: 4.2.1.114, 4.2.1.36]; 4.2.1.114 [EC: 4.2.1.114, 4.2.1.36]; 4.2.1.114 [EC: 4.2.1.114, 4.2.1.36]; 4.2.1.114 [EC: 4.2.1.114, 4.2.1.36]; 4.2.1.114 [EC: 4.2.1.114, 4.2.1.36]

Predicted SEED Role

"Coenzyme B synthesis from 2-oxoglutarate: steps 4, 7, 8, 11, and 12 [large subunit]" in subsystem Coenzyme B synthesis

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.36

Use Curated BLAST to search for 4.2.1.114 or 4.2.1.36

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6LX72 at UniProt or InterPro

Protein Sequence (418 amino acids)

>MMP_RS07610 homoaconitase large subunit (Methanococcus maripaludis S2)
MTLAEKIISKNVGKNVYAKDSVEISVDIAMTHDGTTPLTVKAFEQISDKVWDNEKIVIIF
DHNIPANTSKAANMQVITREFIKKQGIKNYYLDGEGICHQVLPEKGHVKPNMIIAGADSH
TCTHGAFGAFATGFGATDMGYVYATGKTWLRVPETIRVNVTGENENISGKDIILKTCKEV
GRRGATYMSLEYGGNAVHNLSMDERMVLSNMAIEMGGKAGIIEADDTTYRYLENAGVSRE
EILELKKNKITVDESEEDYYKTIEFDITGMEEQVACPHHPDNVKGVSEVEGTELNQVFIG
SCTNGRLNDLRIAAKYLKGKKVNENTRLIVIPASKSIFKEALNEGLIDIFVDSGALICTP
GCGPCLGAHQGVLGDGEVCLATTNRNFKGRMGNTNAQVYLSSPKIAAKSAVKGYITNE