Protein Info for MMP_RS07585 in Methanococcus maripaludis S2

Annotation: isoleucine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1034 TIGR00392: isoleucine--tRNA ligase" amino acids 5 to 837 (833 residues), 998.7 bits, see alignment E=1.2e-304 PF00133: tRNA-synt_1" amino acids 17 to 636 (620 residues), 379.3 bits, see alignment E=5.3e-117 PF09334: tRNA-synt_1g" amino acids 46 to 195 (150 residues), 33.8 bits, see alignment E=4.1e-12 amino acids 583 to 645 (63 residues), 35.9 bits, see alignment 1e-12 PF01406: tRNA-synt_1e" amino acids 568 to 645 (78 residues), 31.7 bits, see alignment 2.7e-11 PF08264: Anticodon_1" amino acids 682 to 827 (146 residues), 98 bits, see alignment E=1.2e-31 PF19302: DUF5915" amino acids 847 to 1028 (182 residues), 73.4 bits, see alignment E=4.8e-24

Best Hits

Swiss-Prot: 100% identical to SYI_METMP: Isoleucine--tRNA ligase (ileS) from Methanococcus maripaludis (strain S2 / LL)

KEGG orthology group: K01870, isoleucyl-tRNA synthetase [EC: 6.1.1.5] (inferred from 100% identity to mmp:MMP1474)

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6LX78 at UniProt or InterPro

Protein Sequence (1034 amino acids)

>MMP_RS07585 isoleucine--tRNA ligase (Methanococcus maripaludis S2)
MKQVKSVNFRELDKKVKEYWKKENTYKKVKALNEHGPEYYFVDGPPYCSGAIHLGTAWNK
IIKDTVLRFKRIQGYNVLDKAGWDMHGLPIEVKVENEFNIGSKKDIETKIGTQEFINKCK
EFALNHLGHMQGQFENLGVWLDFENAYMPIKRDYMEMGWWTLKKAHEKELLTKDLRSGYW
CPRCETSLAEHEVRGEYKEVLDPSVYVKFKLEKSDEYITIWTTTPWTLPSNMLVCVNPEF
DYAYVNVEFENGTAETWIIAEKLVNDVMKKAEKNNDISKFSISKVVKGDSLIGLKYIHPL
LEENEKQQEFAKIENVHTIVPGDHVTLEGGTGLVHTAPGFGEDDFNIGKEHNIPVYAPID
DNGKYTDSIWKGTFVKDMDESVIETLISKNLLVNSGKVKHTYPHCWRCKTPLLFRATEQW
FLSISKIKDSIIEQGKTVDWVPDWVKTRYVNGVSFVGDWNISRQRYWGIPLPIWICEECG
NYEVIGSVDELKERANEKDVDLSDIHKPAVDKITLTCSCGGKMKRTPDVLDVWYDSGLAP
YASIGSKKLKKAQFITEGNDQVTKWFYSQHALSAVVFDDTSYEKCMMHGFTLDETGEKMS
KSLGNIVSPDDVTEQYGADVLRFYLLSANKAWEDLRFSYSEMDETRSMLNTLWNSYAFSA
NYMVLDDFVPNNEYFKHVKDEDAWILSRINTVAKEAVEALEKPHLHVYTWALRDFILNDF
SRWYIKLIRDRTWMEKNDVQKLSAYQTLYYVIMKLISIMAPVTPHLSEEIYQNLKTEDMP
ESIFMNKLTIESEFINETLEKDTEIIREIVDSILKGRDKAKYTLRYPITKITLPENIAET
VEKYGYIIKEQGNVKEIELKEFEGNITVKPNFKELGKIFRSDVPKVVAAINSVAPNELKE
KLKSGNFEVSEYEIKPEYVEFRVEIPENIVGVEFSKGNVYINIEMNDEVIKEGLVREVVR
RIQSMRKDMDLDINEKINVKLEGIDFSSDYLSHIANEVRGNFVESLKSDYNQKWTIKTPN
EETYDISIDIEKNK