Protein Info for MMP_RS07165 in Methanococcus maripaludis S2

Annotation: RNA-splicing ligase RtcB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 480 PF01139: RtcB" amino acids 46 to 480 (435 residues), 617.6 bits, see alignment E=7.3e-190

Best Hits

Swiss-Prot: 100% identical to RTCB_METMP: tRNA-splicing ligase RtcB (rtcB) from Methanococcus maripaludis (strain S2 / LL)

KEGG orthology group: None (inferred from 100% identity to mmp:MMP1392)

MetaCyc: 53% identical to tRNA-splicing ligase (Homo sapiens)
RXN-17942 [EC: 6.5.1.8]; 6.5.1.8 [EC: 6.5.1.8]

Predicted SEED Role

"RNA-2',3'-PO4:RNA-5'-OH ligase"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.5.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6LXF9 at UniProt or InterPro

Protein Sequence (480 amino acids)

>MMP_RS07165 RNA-splicing ligase RtcB (Methanococcus maripaludis S2)
MKSTVTKIAEKTYQLPVSYKDCMRVPGNVYLDELLFENLQEDVFEQIANVACLPGIQKYS
LAMPDCHYGYGFCIGGVAAFDEVTGVISPGGVGFDINCGVRLVKTNLTKNDVTPKLKELL
SEIFKNVPSGLGSKGKIRITKDEIDSVLEEGVSWAVEEEYGWERDIKHIEEHGKMKEADP
TLVSDNAKKRGLPQLGSLGSGNHFLEVQYVDEIFDEEAAKTFGVSPDQVVLMIHTGSRGL
GHQICADYLRYMENAAKKYNIKLPDRQLACAPINSEEGQKYFKAMSCGANYAWANRQLIT
HWIRESFESVFKTSAEDLEMDIIYDVAHNIAKKEQHLVDGVLKNVVVHRKGATRAFGPGH
AEIPSDYVNIGQPVIIPGDMGTASYLMHGTEKAMEQTFGSTAHGAGRALSRVKALKLYTG
NEVQEALQKKGILVMADSKGVIAEECPEAYKDIENVADICHDSGISLKVAKMKPMGVVKG