Protein Info for MMP_RS06010 in Methanococcus maripaludis S2

Annotation: heavy metal translocating P-type ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 723 transmembrane" amino acids 90 to 107 (18 residues), see Phobius details amino acids 113 to 135 (23 residues), see Phobius details amino acids 147 to 167 (21 residues), see Phobius details amino acids 173 to 191 (19 residues), see Phobius details amino acids 325 to 345 (21 residues), see Phobius details amino acids 351 to 373 (23 residues), see Phobius details amino acids 667 to 690 (24 residues), see Phobius details amino acids 697 to 716 (20 residues), see Phobius details PF00403: HMA" amino acids 5 to 65 (61 residues), 54.1 bits, see alignment 2.6e-18 TIGR01511: copper-translocating P-type ATPase" amino acids 128 to 717 (590 residues), 572.8 bits, see alignment E=1.7e-175 TIGR01525: heavy metal translocating P-type ATPase" amino acids 145 to 715 (571 residues), 599.6 bits, see alignment E=1.4e-183 TIGR01494: HAD ATPase, P-type, family IC" amino acids 181 to 687 (507 residues), 312.4 bits, see alignment E=8.9e-97 PF00122: E1-E2_ATPase" amino acids 209 to 386 (178 residues), 194.8 bits, see alignment E=1.5e-61 PF00702: Hydrolase" amino acids 404 to 622 (219 residues), 103.1 bits, see alignment E=3.9e-33

Best Hits

Swiss-Prot: 49% identical to COPA_ARCFU: Probable copper-exporting P-type ATPase (copA) from Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 100% identity to mmp:MMP1165)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6LY28 at UniProt or InterPro

Protein Sequence (723 amino acids)

>MMP_RS06010 heavy metal translocating P-type ATPase (Methanococcus maripaludis S2)
MEVKLKISGMTCAVCVKTIEKSVSKMDGVESIVVNLLDESAVINFDEKFVSIEDIGIKIE
RLGYEVLGIAEEIEELPDKEDELKEKLKKIIVGAVFSIALFSMMYIEIPYKPYLAFLVSL
PPLLYIALPIFKAGFNSFRVKSLNMDVMYSLGMGVAYISALLVTLGLLPMNFMFYDTTIM
LATLLTLGRYLEERAKGRTSEAIKKLMGLQVKTAKVIRNNEELEIPIENVIVGDILLIRP
GEKIAVDGTVFEGDSYVDESMITGEPIPNPKKKGDSVIGGTINKNGILKITAEKIGKDTV
LSQIIQLVKNAQISKPDIQNLADKAVSYFIPVVFTIALISSLYWFFNGGILLAVTTFISV
MVIACPCALGLATPTAITVGVGRGAELGILIKDSKVFDVAGNLKCMIFDKTGTITKGEPE
VDEIISDYSKEEVLLIAGTLEKNSEHPLALAILKKAEELNISLSEPEKFESITGMGIIGT
LKDLRVLIGNRRLMEENNISINEEYNKEISRLEENAKTVIIVGVENKILGIIAISDKIKE
NAKITVQNLREIGIESYMVTGDNEKTAKVIGKEVGILENHVFSNVLPEKKAEIVKSIKEN
AGGYVEFIGDGINDAPALSTADVGIAVGSGTDIAIESGEVVLMNDDLKYVTGFVKLSKRV
LKQIKLNLFWAFAYNSILIPVAAGALYSYNIRFEPELAAFAMTLSSITIIGLSLLLKRYD
PWK