Protein Info for MMP_RS05710 in Methanococcus maripaludis S2

Annotation: ADP-forming succinate--CoA ligase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01016: succinate-CoA ligase, beta subunit
  • amino acids 1 to 360 (360 residues), 430.7 bits, E=2.4e-133
• PF08442: ATP-grasp_2
  • amino acids 2 to 188 (187 residues), 196.9 bits, E=6e-62
• PF13549: ATP-grasp_5
  • amino acids 3 to 204 (202 residues), 34.4 bits, E=3e-12
• PF02786: CPSase_L_D2
  • amino acids 28 to 112 (85 residues), 24.2 bits, E=4.3e-09
• PF00549: Ligase_CoA
  • amino acids 242 to 353 (112 residues), 59.9 bits, E=5.7e-20

Best Hits

Swiss-Prot: 64% identical to SUCC_METJA: Succinate--CoA ligase [ADP-forming] subunit beta (sucC) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

KEGG orthology group: K01903, succinyl-CoA synthetase beta subunit [EC: 6.2.1.5] (inferred from 100% identity to mmp:MMP1105)

Predicted SEED Role

"Succinyl-CoA ligase [ADP-forming] beta chain (EC 6.2.1.5)" in subsystem Serine-glyoxylate cycle or TCA Cycle (EC 6.2.1.5)

MetaCyc Pathways

• incomplete reductive TCA cycle (6/7 steps found)
• pyruvate fermentation to acetate VI (2/3 steps found)
• TCA cycle VIII (Chlamydia) (4/6 steps found)
• pyruvate fermentation to acetate V (1/3 steps found)
• reductive TCA cycle I (6/11 steps found)
• TCA cycle V (2-oxoglutarate synthase) (4/9 steps found)
• adipate biosynthesis (1/5 steps found)
• adipate degradation (1/5 steps found)
• TCA cycle III (animals) (4/10 steps found)
• TCA cycle II (plants and fungi) (3/9 steps found)
• reductive TCA cycle II (5/12 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (12/22 steps found)
• TCA cycle I (prokaryotic) (3/10 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (35/56 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (2/10 steps found)
• superpathway of glyoxylate bypass and TCA (3/12 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (13/26 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (6/17 steps found)
• methylaspartate cycle (3/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• C5-Branched dibasic acid metabolism
• Citrate cycle (TCA cycle)
• Propanoate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.5

Use Curated BLAST to search for 6.2.1.5

Sequence Analysis Tools

See Q6LY86 at UniProt or InterPro

Protein Sequence (362 amino acids)

>MMP_RS05710 ADP-forming succinate--CoA ligase subunit beta (Methanococcus maripaludis S2)
MKLHEYEAKEIFKENNIPIPKNKLVSGKVESIDYPVVIKAQVLVGGRGKAGGILFADNVD
DANEKIESLIGNTVKGELVEKVLLEEQIKIVKEYYVGVVVNRNEKNNVVIFSTEGGVDIE
EVASKTPEKIIKLSLNPEKEFLPYLARGMLKKAGIPSAEIPKVADVLCKVYKAFKNMDGI
LAEINPLVFTEDGKIIAADAVLNVDDDAYFRHNYEKFEEFNKQEKSEFAYVELDGDIAVI
GNGAGLTLASMDIVKEFGGNPACFLDVGGGSNRETVKKALLKATSRKNVKGAFINILGGI
TRCDEVSMGIVDVLKENPNLKFAVRMMGTNEEEGRRILSENGISFESSMEGAAKALTELI
NS