Protein Info for MMP_RS05655 in Methanococcus maripaludis S2

Annotation: phosphoenolpyruvate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01418: phosphoenolpyruvate synthase
  • amino acids 3 to 747 (745 residues), 928.2 bits, E=2.2e-283
• PF01326: PPDK_N
  • amino acids 16 to 331 (316 residues), 410.7 bits, E=7.5e-127
• PF00391: PEP-utilizers
  • amino acids 366 to 435 (70 residues), 97.2 bits, E=5.2e-32
• PF02896: PEP-utilizers_C
  • amino acids 461 to 746 (286 residues), 289.2 bits, E=5e-90

Best Hits

Swiss-Prot: 56% identical to PPSA_PYRHO: Probable phosphoenolpyruvate synthase (ppsA) from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)

KEGG orthology group: K01007, pyruvate, water dikinase [EC: 2.7.9.2] (inferred from 100% identity to mmp:MMP1094)

Predicted SEED Role

"Phosphoenolpyruvate synthase (EC 2.7.9.2)" in subsystem Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 2.7.9.2)

MetaCyc Pathways

• gluconeogenesis II (Methanobacterium thermoautotrophicum) (16/18 steps found)
• glycolysis V (Pyrococcus) (10/10 steps found)
• glycolysis II (from fructose 6-phosphate) (9/11 steps found)
• gluconeogenesis I (10/13 steps found)
• glycolysis I (from glucose 6-phosphate) (10/13 steps found)
• superpathway of anaerobic sucrose degradation (13/19 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (10/17 steps found)
• superpathway of hexitol degradation (bacteria) (10/18 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (10/19 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (35/56 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (13/26 steps found)
• superpathway of N-acetylneuraminate degradation (10/22 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.9.2

Sequence Analysis Tools

See Q6LY97 at UniProt or InterPro

Protein Sequence (758 amino acids)

>MMP_RS05655 phosphoenolpyruvate synthase (Methanococcus maripaludis S2)
MKLITWLDDLRNDDVDIAGGKGASLGEMWNAKLPVPPAFVVTSEAYRYFIKETKLQDEIE
RILSNIDVNNNEELNKASKEVRYAIENVKMPDDLKLSIIESYHKMCELNGDDETFVAVRS
SATAEDLPDASFAGQQETFLNMKGNEQVLQAVQECFSSLFTPRAIFYREEKGFDHFEVAL
AAVVQKMVNADQAGVMFTVNPINQDYNQMVIEGAWGLGEGVVSGTVSPDTYIVAKDTQEV
LSVNVARKDTMFIKDADGVTKEIKTPEDLIEKQVLSEEMIKKLENVGQTIENHYKRPMDI
EWSIENGEIYMLQARPITTLTDKKDSKSSNEIEGKIILKGIGASPGVESGKVKIISEIEE
IGRILDGDILVTKMTTPDMVPAMKKAAAIITDDGGLTCHAAIISRELGTPCVVGTKGATK
NLKDEECVTVDGEKGIVYEGKVGKPEEKAPAQVVYAGAPVITATEIKANVSMPEVAERAA
ATGADGVGLLRAEHMILGTGVHPGKILKEQGEGALTEVFAEGIRKVADAFYPRPVTYRTL
DAPTDEFVGLKGGEDEPHEHNPMMGWRGIRRGLDQSEILKCELKAIKKLREEGYRNINVM
IPLVTNVDEIRKVKEIANEVGLKLRLDVEFGVMVETPAAALIIDDIIEEGIDFISLGTND
LTQYTIAIDRNNENVSKYYLENHPAVLKLIELVITSCKRAGVKTSICGQAGSRPQIVEKL
VEWGITSISANIDAVETIRNVVARTEQKIILESIRKQK