Protein Info for MMP_RS05240 in Methanococcus maripaludis S2

Annotation: carbamoyl-phosphate synthase large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01369: carbamoyl-phosphate synthase, large subunit
  • amino acids 2 to 1062 (1061 residues), 1569.8 bits, E=0
• PF02786: CPSase_L_D2
  • amino acids 127 to 332 (206 residues), 262 bits, E=1.6e-81
  • amino acids 682 to 884 (203 residues), 125.6 bits, E=9.2e-40
• PF07478: Dala_Dala_lig_C
  • amino acids 157 to 300 (144 residues), 26.2 bits, E=2.5e-09
  • amino acids 705 to 852 (148 residues), 34.9 bits, E=5.4e-12
• PF02787: CPSase_L_D3
  • amino acids 424 to 505 (82 residues), 75 bits, 1.7e-24
• PF02655: ATP-grasp_3
  • amino acids 689 to 854 (166 residues), 23.5 bits, E=2.4e-08
• PF02222: ATP-grasp
  • amino acids 689 to 854 (166 residues), 38.7 bits, E=3.7e-13
• PF02142: MGS
  • amino acids 965 to 1051 (87 residues), 73.6 bits, E=5.3e-24

Best Hits

Swiss-Prot: 62% identical to CARB_ARCFU: Carbamoyl-phosphate synthase large chain (carB) from Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)

KEGG orthology group: K01955, carbamoyl-phosphate synthase large subunit [EC: 6.3.5.5] (inferred from 60% identity to ave:Arcve_1538)

Predicted SEED Role

"Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (15/18 steps found)
• superpathway of histidine, purine, and pyrimidine biosynthesis (35/46 steps found)
• UMP biosynthesis II (6/6 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (8/9 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• L-citrulline degradation (3/3 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• superpathway of arginine and polyamine biosynthesis (13/17 steps found)
• UMP biosynthesis I (5/6 steps found)
• UMP biosynthesis III (5/6 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-arginine degradation V (arginine deiminase pathway) (3/4 steps found)
• L-citrulline biosynthesis (4/8 steps found)
• L-glutamate and L-glutamine biosynthesis (3/7 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)
• superpathway of L-citrulline metabolism (6/12 steps found)
• allantoin degradation IV (anaerobic) (2/9 steps found)
• guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Sequence Analysis Tools

See Q6LYH8 at UniProt or InterPro

Protein Sequence (1081 amino acids)

>MMP_RS05240 carbamoyl-phosphate synthase large subunit (Methanococcus maripaludis S2)
LKREDIKKVMILGSGPIVIGQAAEFDFSGSQACKSLKEEGIYTILVNSNPATIQTDTNIA
DKVYLEPLNPKILEKIIEKEQPDAILPTMGGQTGLNLAMELSKRGILEKHGVELLGSTES
VIETSEDRDLFNKAMEEINQPIAKSAAVHSVEEAIGATKELGYPAIVRPAFTLGGTGGGI
ANNEEELIEITKKGLKYSMIKQVLIDQSLLGWKEYEYEVMRDKNDTCIVVCNMENIDPMG
IHTGESIVTAPSQTLSDEFHQKLRDASLQIIRHLKIEGGCNVQFAVNPEMTDYVVIEVNP
RVSRSSALASKATGYPIAKIAAKIAIGRTLDEIQNDVTKETPASFEPTIDYVVVKIPRWP
FDKFRTVDKKLGTSMKSTGEIMAIGRNLEEALQKAVRSLDIGRFGIIADGKDKEYSNSEI
VDILEHATDERLFVIAYALDKGWSVDGICERTGINPFFIEKIKKIIDCKKELEVISRIPV
DDEKLKEILLKAKSLGFSDVQISKIFSKTENEIRDLRKRLEVIPVYKMVDTCAAEFEAKT
PYYYSAYERYFDEEQNESVSSDRKKVIILGSGPIRIGQGVEFDYSTVHAIFALKELGIEA
IIVNNNPETVSTDYDTSDKLYFEPLVYEEIMNIIENENKNGQLLGVIVQFGGQTAINLAM
KLYNSGVNILGTSPQSIDLAEDRDQFIHVLEKLKIPQADGATAFSEEQALKVVERIGYPA
LVRPSYVLGGRAMQIVYNTEDLKDYMREAVKVSSDHPILIDKFLEEAVEVDVDAVCDGES
VFIGAIMEHIEEAGIHSGDSACVIPPQTLSKEVIEKIAEHTTKLALELGVIGLLNIQYAV
KDGVVYIIEANPRASRTIPYVSKSVGVPLAKIATNAIMGKKLKEMGYFGLAKSKYVSVKE
AVFPFLKLPGVDPVLSPEMKSTGEAIGIDQDFGKAFYKSQLSANMELPTSGTVFISVRNR
DKDNITKIAKKYHNLGFEIVATRGTARELRLFDIPVREVRKISESMQNSVLDLMQKGEVD
LIINTSSGDKAKTDGYFIRRAAVELNIPCMTTLQGAYAAIKAIEAIKSGELGVYSLNELE
N