Protein Info for MMP_RS05120 in Methanococcus maripaludis S2

Annotation: DNA topoisomerase VI subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 664 TIGR01052: DNA topoisomerase VI, B subunit" amino acids 16 to 651 (636 residues), 717.6 bits, see alignment E=3.1e-220 PF02518: HATPase_c" amino acids 41 to 154 (114 residues), 47.1 bits, see alignment E=4.6e-16 PF13589: HATPase_c_3" amino acids 42 to 164 (123 residues), 36 bits, see alignment E=1e-12 PF09239: Topo-VIb_trans" amino acids 462 to 629 (168 residues), 178.6 bits, see alignment E=1.2e-56

Best Hits

Swiss-Prot: 66% identical to TOP6B_METJA: Type 2 DNA topoisomerase 6 subunit B (top6B) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

KEGG orthology group: K03167, DNA topoisomerase VI subunit B [EC: 5.99.1.3] (inferred from 100% identity to mmp:MMP0989)

Predicted SEED Role

"DNA topoisomerase VI subunit B (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6LYK2 at UniProt or InterPro

Protein Sequence (664 amino acids)

>MMP_RS05120 DNA topoisomerase VI subunit B (Methanococcus maripaludis S2)
MAEDTNAVVEESLFDEFKEHSISEFFRKNRHMLGYSGRLRSMTTIVHELVTNSLDACEES
EILPEITVEIKKIGSETFGVNIEDNGPGIPPEFVPKVFGKMLAGSKLHRLVQSRGQQGIG
AAGVLLFAQMTTGKPLKITTSTGNGTIYEMEIKMSIEKNEGDVVSRNTREGFWRGTRVEG
EFKDVTYNRREQGPFEYLRRISLSAPHAKITLEDPEETIVFERTVNDIPERPEVMKPHPY
GLTTDELLHISRVTDSSRVSSMLNSELSRVTMKRIKELEEYVLRDTLLENYRSSVFWDTI
VSCYLNFDFTKYFDIYGHYFDKKEIEDVKQLINGLPEGLGELKTYCMKYLVTQYLVQKLD
DEEIKEIKNHFKKKPENFMEYMDKKYLNASLLDEFRRKLKNITKSPAEFVKSLVDLAMVS
DTELEKYRKAIRDLLKKNPKEMTWKDSEVIVNVLQDMDFMAPSTVGLRPIGEENIEKSLN
TLEPEFLKTLTRKPKTYKGGVPFAVEVGLAYGGEAGRSGDESRKMEIMRFSNHVPLLFDT
SGCGLTNAVKSVNWRRYGLRNDEDAPVTVFVNLISTHIPYTSAGKQAIASSNEENEEIFN
EIRQSLMICARELEKHISKLRKEKEEEQKRKYVMKYAVIFAEGLASITGRPKEEIEANVV
NLLR