Protein Info for MMP_RS05025 in Methanococcus maripaludis S2

Annotation: ATP-dependent DNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 573 PF04675: DNA_ligase_A_N" amino acids 3 to 178 (176 residues), 130.5 bits, see alignment E=1.1e-41 TIGR00574: DNA ligase I, ATP-dependent (dnl1)" amino acids 62 to 567 (506 residues), 446.6 bits, see alignment E=6.2e-138 PF01068: DNA_ligase_A_M" amino acids 228 to 440 (213 residues), 184.9 bits, see alignment E=2.3e-58 PF04679: DNA_ligase_A_C" amino acids 460 to 551 (92 residues), 59.1 bits, see alignment E=7.4e-20

Best Hits

Swiss-Prot: 100% identical to DNLI_METMP: DNA ligase (lig) from Methanococcus maripaludis (strain S2 / LL)

KEGG orthology group: K10747, DNA ligase 1 [EC: 6.5.1.1] (inferred from 100% identity to mmp:MMP0970)

Predicted SEED Role

"ATP-dependent DNA ligase (EC 6.5.1.1)" in subsystem DNA Repair Base Excision or DNA replication, archaeal (EC 6.5.1.1)

Isozymes

Compare fitness of predicted isozymes for: 6.5.1.1

Use Curated BLAST to search for 6.5.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6LYM1 at UniProt or InterPro

Protein Sequence (573 amino acids)

>MMP_RS05025 ATP-dependent DNA ligase (Methanococcus maripaludis S2)
MLFSDFCKILDKIEKTTKRLEKTDYFVELIDFIKTSEKPENLKQVSQITIGRVFAEFENK
EIGIGPNLLLEAVKTTGIPEKDLKSKIKETGDIGTAVENLSSNIKQVSLFSQALTLEEVY
STLKKLSEIEGNSSQKKKTRIISNLLILADPVESRYISRLILEDMRIGMNIPTILASFSN
YFNVNKESVEKIYAVTNDIGLLGEKLISGSDIENDPELKLKVFRPIKPMLAQLTPSIEDA
MIETKMPQFETKYDGARVQVHKSNGDVKIYSRRLENITNSVPELVEEIKKLDIDNIILEG
ECVAMDLDSGKPRPFQDILRRFRRKYNIDKMAEKIALRIYFFDVLYYNRGLIDTPLKTRR
EILEKLFGTNNWDSELEKIKKEIFSSKMLFSSFKLNSGDPNLVKEFFNWSLSIGHEGIMI
KNPDAPYTPGSRVKTMYKVKPTLENLDVVVTRAKIGMGKRKDWYGSYELSVKDNDGNLHV
IGNVGSGLTEDDLEKLTKIVNEIKIEDLGEEVILEPKIVLEVTYEEIQTSEKYEMGYALR
FPRVVQIREDKSINDINTLDDVKKIYEIERNRK