Protein Info for MMP_RS04970 in Methanococcus maripaludis S2

Annotation: F420-dependent thioredoxin reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 PF07992: Pyr_redox_2" amino acids 3 to 287 (285 residues), 161.8 bits, see alignment E=7.5e-51 PF00890: FAD_binding_2" amino acids 4 to 41 (38 residues), 23.6 bits, see alignment 8.3e-09 PF13738: Pyr_redox_3" amino acids 53 to 271 (219 residues), 87.6 bits, see alignment E=2.7e-28 PF00070: Pyr_redox" amino acids 143 to 215 (73 residues), 38 bits, see alignment E=5.9e-13

Best Hits

Swiss-Prot: 64% identical to TRXB_METJA: Putative thioredoxin reductase (MJ1536) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

KEGG orthology group: K00384, thioredoxin reductase (NADPH) [EC: 1.8.1.9] (inferred from 100% identity to mmp:MMP0959)

Predicted SEED Role

"Thioredoxin reductase (EC 1.8.1.9)" in subsystem Glycine reductase, sarcosine reductase and betaine reductase or Thioredoxin-disulfide reductase or Wyeosine-MimG Biosynthesis (EC 1.8.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.8.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6LYN1 at UniProt or InterPro

Protein Sequence (300 amino acids)

>MMP_RS04970 F420-dependent thioredoxin reductase (Methanococcus maripaludis S2)
MVYDLIIIGGGPAGLTAGIYAMRAKLSTLCLEKQNEGGKIAEAGIVENYPGFESIKGFEL
AQKFSEHAKHFELPIIHEEVEKIDTSSKPYKVITKHETYEAKSIVIASGSRYKKPGLNED
DFMGKGVCYCVMCDAFFFLNKEVIVLGRGTSAIMAAYNLKDIAKKITIVTDRSELKAVEK
IMEDRMNLMNNLEIVLNAEPIEIVGKEKAEGVKVSIDGKEKIISADGIFISFGYVPNTEF
LDGSEIKLNKRKFIETDENCKTSVEGIYACGDVTGGILQVSKAVGEGVTAFTGALSSIQK