Protein Info for MMP_RS04950 in Methanococcus maripaludis S2

Annotation: succinate--CoA ligase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01019: succinate-CoA ligase, alpha subunit
  • amino acids 2 to 286 (285 residues), 429.3 bits, E=3.7e-133
• PF02629: CoA_binding
  • amino acids 5 to 98 (94 residues), 94.4 bits, E=1.2e-30
• PF13607: Succ_CoA_lig
  • amino acids 144 to 278 (135 residues), 43.2 bits, E=6.9e-15
• PF00549: Ligase_CoA
  • amino acids 150 to 270 (121 residues), 88.4 bits, E=9.1e-29

Best Hits

Swiss-Prot: 72% identical to SUCD_METJA: Succinate--CoA ligase [ADP-forming] subunit alpha (sucD) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

KEGG orthology group: K01902, succinyl-CoA synthetase alpha subunit [EC: 6.2.1.5] (inferred from 100% identity to mmp:MMP0955)

MetaCyc: 52% identical to succinyl-CoA synthetase subunit alpha (Escherichia coli K-12 substr. MG1655)
Succinate--CoA ligase (ADP-forming). [EC: 6.2.1.5]

Predicted SEED Role

"Succinyl-CoA ligase [ADP-forming] alpha chain (EC 6.2.1.5)" in subsystem Serine-glyoxylate cycle or TCA Cycle (EC 6.2.1.5)

MetaCyc Pathways

• incomplete reductive TCA cycle (6/7 steps found)
• pyruvate fermentation to acetate VI (2/3 steps found)
• TCA cycle VIII (Chlamydia) (4/6 steps found)
• pyruvate fermentation to acetate V (1/3 steps found)
• reductive TCA cycle I (6/11 steps found)
• TCA cycle V (2-oxoglutarate synthase) (4/9 steps found)
• adipate biosynthesis (1/5 steps found)
• adipate degradation (1/5 steps found)
• TCA cycle III (animals) (4/10 steps found)
• TCA cycle II (plants and fungi) (3/9 steps found)
• reductive TCA cycle II (5/12 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (12/22 steps found)
• TCA cycle I (prokaryotic) (3/10 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (35/56 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (2/10 steps found)
• superpathway of glyoxylate bypass and TCA (3/12 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (13/26 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (6/17 steps found)
• methylaspartate cycle (3/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• C5-Branched dibasic acid metabolism
• Citrate cycle (TCA cycle)
• Propanoate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.5

Use Curated BLAST to search for 6.2.1.5

Sequence Analysis Tools

See Q6LYN5 at UniProt or InterPro

Protein Sequence (287 amino acids)

>MMP_RS04950 succinate--CoA ligase subunit alpha (Methanococcus maripaludis S2)
LILLDENTRAIVQGITGNQGRFHTKNMLECKTNLLAGVTPGKGGQDVYGVPVYDTVLETV
EKYDANASLIFIPAPFVKDAAYEAIDAGVELVTIITEHIPIQDSMDIVAYGKKHGVNIIG
PNTPGLASPKVGKLGIIPMSILKEGNIGMVSRSGTLTYEIASQLTLSNYGQSSCVGIGGD
PVTGLRYIEVLEMFENDPETDAVVMVGEIGGNAEEDAAKKMISKMKKPVISYIAGQSAPE
GKRMGHAGAIIEKGVGTAKSKMQALENAGAHVAKRISDIPLLLKEII