Protein Info for MMP_RS04270 in Methanococcus maripaludis S2

Annotation: coenzyme F420 hydrogenase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 410 TIGR03295: coenzyme F420 hydrogenase, subunit alpha" amino acids 1 to 410 (410 residues), 723.7 bits, see alignment E=3.2e-222 PF00374: NiFeSe_Hases" amino acids 41 to 117 (77 residues), 54.7 bits, see alignment E=4.6e-19 amino acids 279 to 393 (115 residues), 27.9 bits, see alignment E=6e-11

Best Hits

Swiss-Prot: 89% identical to FRHA_METVO: Coenzyme F420 hydrogenase subunit alpha (frhA) from Methanococcus voltae

KEGG orthology group: K00440, coenzyme F420 hydrogenase alpha subunit [EC: 1.12.98.1] (inferred from 100% identity to mmp:MMP0820)

Predicted SEED Role

"Coenzyme F420 hydrogenase alpha subunit (FrcA) (EC 1.12.98.1)" in subsystem Coenzyme F420 hydrogenase or NiFe hydrogenase maturation (EC 1.12.98.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.12.98.1

Use Curated BLAST to search for 1.12.98.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6LZ09 at UniProt or InterPro

Protein Sequence (410 amino acids)

>MMP_RS04270 coenzyme F420 hydrogenase subunit alpha (Methanococcus maripaludis S2)
MGKTVEINPTTRHEGHTKLVLKVDDDGIVEKGNYLSVTPVRGFEKFLVGKVAEFAPIAVS
RFCGICPIAHATSSVEAIEDACGITPPKDGLLLRELTGLGNKMHSHPLHEFLIAPDFIPE
ADRVEYIKRIQEMRKTGQFIVDTIGGEAIHAPNIKVGGMLKNITENAASKIYYKCREYEK
LAKEHVEYLIPIFESNMLADGTEIPEKLGYHDFGYIATDATYGNRENIDQSKVVEYTPYD
VYEKDVAVQACTTIPRYNGRLMEVGPRARFAKFHDFKNKGAMAIHIARAYENVVHVKRAM
EIIDELDVNGKTRSNEPILGDGEKIGLGVHEAARGHNTHQAAIDAKGRITYYNAIVATTW
NIPLIGKATEGTHYKFAEHVVRAYDPCVSCATHVIAMDYDNKIVDKKIIQ