Protein Info for MMP_RS03650 in Methanococcus maripaludis S2

Annotation: leucine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 955 transmembrane" amino acids 83 to 98 (16 residues), see Phobius details TIGR00395: leucine--tRNA ligase" amino acids 19 to 955 (937 residues), 1257.7 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 23 to 525 (503 residues), 110.7 bits, see alignment E=1.5e-35 amino acids 589 to 683 (95 residues), 26 bits, see alignment E=7.9e-10 PF09334: tRNA-synt_1g" amino acids 45 to 118 (74 residues), 36.4 bits, see alignment 5.4e-13 amino acids 607 to 692 (86 residues), 38.3 bits, see alignment E=1.4e-13 PF13603: tRNA-synt_1_2" amino acids 223 to 350 (128 residues), 40.8 bits, see alignment E=3.4e-14 PF08264: Anticodon_1" amino acids 722 to 850 (129 residues), 90.2 bits, see alignment E=2.5e-29

Best Hits

Swiss-Prot: 100% identical to SYL_METMP: Leucine--tRNA ligase (leuS) from Methanococcus maripaludis (strain S2 / LL)

KEGG orthology group: K01869, leucyl-tRNA synthetase [EC: 6.1.1.4] (inferred from 100% identity to mmp:MMP0697)

Predicted SEED Role

"Leucyl-tRNA synthetase (EC 6.1.1.4)" (EC 6.1.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6LZD2 at UniProt or InterPro

Protein Sequence (955 amino acids)

>MMP_RS03650 leucine--tRNA ligase (Methanococcus maripaludis S2)
MDQKEVIGETGHKSVDLIKIMDKWQKKWTEAKIFEAEHDSRDKFFITAAFPYLNGVLHAG
HLRTFTIPETIARYQRMKNKNVLWTFGFHVTGTPILGLANQIKERKEDIIWAYNNLHNIP
MDELLKLNTPEAIVECFSKKATEAFKRMGFSLDWRRNFKTDDKVFSKFIEWQFYKLKEMG
HITKGSHPVRYCPKCENPVEDHDLLHGEESTTVEYSLIKFTSKFDGKEIIMPMATLRPET
VFGVTNAWVNPNEIYVMAEVHDEIQKLDSEDVDLKYNGIWIIGKECADKLKEQDRKIEIL
KEIKGSELLGLKIKNPVTKKEVPLLPADFVEMGIGTGCVMSVPAHAPYDYVALRDLGKVE
EVGLIPLIEIEGYDKYPAKEIVEKLGIKDQNDEELLEQATSKIYKDEFHKGKLNENCGEY
TGISVKDIKEKLTKDYINSNIAEIMYEFSEQKVVCRCGEKCIIKTVKGQWFINYSDENWK
KLAHECIDNMNFAPEGIRQEFHNKVDWMKDKACARKRGLGTILPFDENWIIESLSDSTIY
MAYYTIARFINEGLTPEQLIPELFDYVYLGNGNAEEIAKNSKIQAETIEEMRKEFLYYYP
LDWRCSAKDLIPNHLTFMIFNHVALFGREHWPRGIEINGYVTIEGKKLSKSKGPVLPVSE
VAENFGADVARFYITTCAELPQDADVKFKEMEKARDNLIKLYDLAVSVMEEESAEKELSL
IDKWLLHKTYSSINSAETAYEEFQLRKIGLMFYELINDLRWYKRRGGENNSVLKEVVEIW
TKLLSPVTPHLCEEIWEKLGYAGFISQEMYPVSKPELINEDLELGEEFIKSAMEDIRNIK
GVAKINPEKMYLYTADDWKYDLLEFMNENAEKNVKALIPMVMKEDKFKRHGKEVMKLINE
IMKIGVKKAIAEVEILENAKTFIESEFDCKVIVNGEDVKGKKKFAIPYKPAIYME