Protein Info for MMP_RS03210 in Methanococcus maripaludis S2

Annotation: tRNA guanosine(15) transglycosylase TgtA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 649 TIGR00449: tRNA-guanine family transglycosylase" amino acids 2 to 342 (341 residues), 318.3 bits, see alignment E=5.8e-99 TIGR00432: tRNA-guanine(15) transglycosylase" amino acids 2 to 647 (646 residues), 906 bits, see alignment E=1.3e-276 PF01702: TGT" amino acids 11 to 342 (332 residues), 309 bits, see alignment E=6.8e-96 PF14810: TGT_C2" amino acids 502 to 572 (71 residues), 72.5 bits, see alignment E=4e-24 PF01472: PUA" amino acids 575 to 645 (71 residues), 51.3 bits, see alignment E=1.4e-17

Best Hits

Swiss-Prot: 100% identical to ATGT_METMP: tRNA-guanine(15) transglycosylase (tgtA) from Methanococcus maripaludis (strain S2 / LL)

KEGG orthology group: K00773, queuine tRNA-ribosyltransferase [EC: 2.4.2.29] K07398, conserved protein with predicted RNA binding PUA domain (inferred from 100% identity to mmp:MMP0610)

MetaCyc: 69% identical to 7-cyano-7-deazaguanine tRNA-ribosyltransferase monomer (Methanocaldococcus jannaschii)
RXN-12144 [EC: 2.4.2.48]

Predicted SEED Role

"archaeosine tRNA-ribosyltransferase (EC 2.4.2.-) type 1 / archaeosine tRNA-ribosyltransferase (EC 2.4.2.-) PUA domain" in subsystem Queuosine-Archaeosine Biosynthesis (EC 2.4.2.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.-

Use Curated BLAST to search for 2.4.2.- or 2.4.2.29 or 2.4.2.48

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6LZL5 at UniProt or InterPro

Protein Sequence (649 amino acids)

>MMP_RS03210 tRNA guanosine(15) transglycosylase TgtA (Methanococcus maripaludis S2)
MFEIKARDAMGRLGSITINGKKIETPTIMPVIHPNPKKQTVSMDLINKMADVVITNSYIT
YTTPELREIAETKGIHELIDFKNVVVTDSGSFQLSVYGDVNVGPMEIIDFQEKIGVDVGT
ILDIPTGPDVSREKAESDLIETFKRAEDSIKRRKEMGYKLALNGTIQGSKYLDLRQKSAE
VMGKMDFDIYPIGAVVPLMEDYRYREVAEVILNSKMHLPTNKPVHLFGCGHPMLFALSVA
LGCDLFDSAAYALYAKNGRYLTADGTLHLKDMKDLKSFPCTCKVCSEYTPKQLYNLEEKE
KTRLLAEHNLYVTFEEIDRIKNAIKEGNLWELVEERCRSHPKLLNGLRVISKYMDFIEKH
DPVSKKSGFFYTGYESMNRPEIYRHKQRLDRIQYDKIYVTSVSENTSKPYSENLSNVPCD
VDVLVKDSVFGLVPLNIDTMYPLAQNEVPDLYDFEKKYNNEFVSEFNEKHAEKILDISTY
NYYINHYGKKKECDKINPDIFRIGKMLEYQYGAKILDEELMEKVKSRRSKNTGRIRNLLL
EKEVLFTLRANDNFLIPAKSGAELLHEKLEFPKYRIVIDSSVEEFARAGKSVYSKFVKDC
DPELRPFEEVLIVNSDDELLAYGTTILNGRELMEFDYGVAATLRGGLKK