Protein Info for MMP_RS02205 in Methanococcus maripaludis S2

Annotation: threonine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 622 PF08915: tRNA-Thr_ED" amino acids 1 to 135 (135 residues), 179.6 bits, see alignment E=4.9e-57 TIGR00418: threonine--tRNA ligase" amino acids 22 to 600 (579 residues), 593.1 bits, see alignment E=3.6e-182 PF00587: tRNA-synt_2b" amino acids 275 to 484 (210 residues), 57.3 bits, see alignment E=3.4e-19 PF03129: HGTP_anticodon" amino acids 505 to 599 (95 residues), 86.9 bits, see alignment E=1.3e-28

Best Hits

Swiss-Prot: 100% identical to SYT_METMP: Threonine--tRNA ligase (thrS) from Methanococcus maripaludis (strain S2 / LL)

KEGG orthology group: K01868, threonyl-tRNA synthetase [EC: 6.1.1.3] (inferred from 100% identity to mmp:MMP0414)

Predicted SEED Role

"Threonyl-tRNA synthetase (EC 6.1.1.3)" (EC 6.1.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6M057 at UniProt or InterPro

Protein Sequence (622 amino acids)

>MMP_RS02205 threonine--tRNA ligase (Methanococcus maripaludis S2)
MKTLLIHSDYLEFEAKEKTKVAEKTDVLNGKMDECLTVFIAVEKDDESDPNAVVKNAVEE
IIKTADNLKVKNVVVYPYAHLSSDLGSPATAKEILAEIESELSNNYEVLRAPFGWYKSFK
ISCKGHPLSELSRKITTDRKEEVKKEKVVSKFYIIDGESQNLTEVNDEIIAKMEDKRLLA
LLKHELEIKEEATERGEPPHVKYIKEKEICDYEPSSDAGHFRWYPKGKLIRDLLSDYVYN
LVVERGGMPVETPVMYDLQNNAIREHADKFGERQYRFTQGGKDLMLRFAACFGQFMMKKD
MYLLPKHLPLRLYELSTYSFRYEQRGELVGLKRLRAFTMPDMHTVCMDMKQAMEAFEDQL
WMGLKTGDDFKTPYAIIFRFTQDFFDENKEWFFAMAKEYKQKYGKDAILEILSGRKHYWV
GKVDMAVVDSFGRPIENPTVQIDVESAERFGIVVHDGDKKVHPIILHCSPTGSVERVLCG
LLENAYLNTLENKPPALPTWLTPIQARVIPVGDKHEEYALEVVNKLRSSGIRADFDDREE
SMGKKIRNAGTDWVNYVVVIGDSEMESGKLTVTVREESELKKPKKESLSVEELIEKISSE
TIGAPKRPLPLPMKCSMQPIFR