Protein Info for MMP_RS02030 in Methanococcus maripaludis S2

Annotation: homoaconitase small subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 162 TIGR02087: 3-isopropylmalate dehydratase, small subunit" amino acids 6 to 160 (155 residues), 213.5 bits, see alignment E=6.7e-68 PF00694: Aconitase_C" amino acids 40 to 107 (68 residues), 76.7 bits, see alignment E=1.1e-25

Best Hits

Swiss-Prot: 62% identical to HACB_METJA: Methanogen homoaconitase small subunit (hacB) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

KEGG orthology group: K01705, homoaconitate hydratase [EC: 4.2.1.36] (inferred from 100% identity to mmp:MMP0381)

MetaCyc: 62% identical to homoaconitase beta subunit (Methanocaldococcus jannaschii)
Methanogen homoaconitase. [EC: 4.2.1.114, 4.2.1.36]; 4.2.1.114 [EC: 4.2.1.114, 4.2.1.36]; 4.2.1.114 [EC: 4.2.1.114, 4.2.1.36]; 4.2.1.114 [EC: 4.2.1.114, 4.2.1.36]; 4.2.1.114 [EC: 4.2.1.114, 4.2.1.36]; 4.2.1.114 [EC: 4.2.1.114, 4.2.1.36]

Predicted SEED Role

"Coenzyme B synthesis from 2-oxoglutarate: steps 4, 7, 8, 11, and 12 [small subunit]" in subsystem Coenzyme B synthesis

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.36

Use Curated BLAST to search for 4.2.1.114 or 4.2.1.36

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6M090 at UniProt or InterPro

Protein Sequence (162 amino acids)

>MMP_RS02030 homoaconitase small subunit (Methanococcus maripaludis S2)
MMKITGKVHLFGDDIDTDAIIPGAYLKTTDEYELASHCMAGIDENFPERVEDGDFLVAGE
NFGCGSSREQAPIAIKYCGIKAIIVESFARIFYRNCINLGVFPIECKGISKHVKDGDVIE
LDLEEKKVILKDTVLDCNLPTGTAKDIMDEGGLINYAKKQKN