Protein Info for MMP_RS01740 in Methanococcus maripaludis S2

Annotation: methionine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 663 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details transmembrane" amino acids 478 to 482 (5 residues), see Phobius details PF09334: tRNA-synt_1g" amino acids 3 to 387 (385 residues), 538.7 bits, see alignment E=1.9e-165 TIGR00398: methionine--tRNA ligase" amino acids 4 to 520 (517 residues), 624.9 bits, see alignment E=1.3e-191 PF00133: tRNA-synt_1" amino acids 11 to 238 (228 residues), 72.5 bits, see alignment E=7.6e-24 PF01406: tRNA-synt_1e" amino acids 16 to 127 (112 residues), 33.9 bits, see alignment E=5.7e-12 PF19303: Anticodon_3" amino acids 406 to 531 (126 residues), 24.1 bits, see alignment E=7.6e-09 TIGR00399: methionine--tRNA ligase, beta subunit" amino acids 530 to 662 (133 residues), 145.5 bits, see alignment E=8.7e-47 PF01588: tRNA_bind" amino acids 569 to 661 (93 residues), 101.2 bits, see alignment E=7.2e-33

Best Hits

Swiss-Prot: 100% identical to SYM_METMP: Methionine--tRNA ligase (metG) from Methanococcus maripaludis (strain S2 / LL)

KEGG orthology group: K01874, methionyl-tRNA synthetase [EC: 6.1.1.10] (inferred from 100% identity to mmp:MMP0326)

Predicted SEED Role

"Methionyl-tRNA synthetase (EC 6.1.1.10)" (EC 6.1.1.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6M0E5 at UniProt or InterPro

Protein Sequence (663 amino acids)

>MMP_RS01740 methionine--tRNA ligase (Methanococcus maripaludis S2)
MRHLVTTALAYTNGPLHLGHARSTYIPADIFTRYLKLKGEEVIHVGGTDNHGVPITLTAE
KEGVKPIDIVDRYHNAIKADLDSLNICFDNFGRTHSDIHIETAQEFYSKLKENGYIYEKE
IEQFYCEKCDMYLADRYVEGICPFCEGEARGDHCEVCGRHLEPTELVNPYCIHCNSKPEI
KRTTHYFFKLSAMQDSLKEYIENSPEMPEHVKNMALRWIEELHDWDVSRNIKWGVPIPGC
DDQVMYVWIEAPIGYVSFTKQLGDIWKSYWLENTDDSKISHFIGKDITVHHAVFWPGILK
GIGGYKMPNAVVSGGYLTLENKKMSTSKNWVVWVKDFVENFSSDYLRYFFMINAPLNRDT
DFSWDDFQKRINTELIDIIGNFTHRTLVFTERKFGSTPIVDLNQLKDEDKKLISKCDDTE
KLVDSLIREYNFKDALMEIIHLAKEGNGYFQGMAPWAIKNEERLKEVMYTCSVVLKYIIY
LLSSFMPEKTALLLEYMNEELDLKVRGNPLKKPKVIFTKVSDEDISRMKENLMKATKKAE
TKSDEKSKKVKSGEKMDIIDIDYFGNIDLRVGQILEVEEVPRSKKLYKIIADLGDEKRQI
VSGLIGAYDAEELVGKKVIIICNLKPAKLCGVESQGMLLAAEDDSIVSLLSLDRDLPVGS
KIH