Protein Info for MMP_RS01450 in Methanococcus maripaludis S2

Annotation: tRNA pseudouridine(38-40) synthase TruA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 262 TIGR00071: tRNA pseudouridine(38-40) synthase" amino acids 3 to 218 (216 residues), 144.9 bits, see alignment E=1.6e-46 PF01416: PseudoU_synth_1" amino acids 120 to 222 (103 residues), 77.2 bits, see alignment E=6.4e-26

Best Hits

Swiss-Prot: 100% identical to TRUA_METMP: tRNA pseudouridine synthase A (truA) from Methanococcus maripaludis (strain S2 / LL)

KEGG orthology group: K06173, tRNA pseudouridine synthase A [EC: 5.4.99.12] (inferred from 100% identity to mmp:MMP0268)

Predicted SEED Role

"tRNA pseudouridine synthase A (EC 4.2.1.70)" in subsystem tRNA processing (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6M0K2 at UniProt or InterPro

Protein Sequence (262 amino acids)

>MMP_RS01450 tRNA pseudouridine(38-40) synthase TruA (Methanococcus maripaludis S2)
MYIFKIAYDGKLSFQTQPHGETVCDKISNALLDCGYLDNKDRVPLYHGGRTDRGVAALGN
YVVYEMDKKPVLPRVQSKLKWDGIWVLGCKKIEIFPEIEHRHYQYTLPNKNHDVELMKKA
SEKLIGTHYFQNLSKRDKTKVKDPVRTLYDIKISSNDYFITIDIFGESFLWNMVRRIIRL
LSDIGKHKIENPEKFIELILSEDYKKGYPPSPAEGLILVDVKTNIDIDLDSYVIRNLKNS
WEKSLNNSLMRLGLCKTVLSKT