Protein Info for MMP_RS01045 in Methanococcus maripaludis S2

Annotation: diphthamide biosynthesis enzyme Dph2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 309 TIGR00322: diphthamide biosynthesis enzyme Dph1/Dph2 domain" amino acids 3 to 292 (290 residues), 233.4 bits, see alignment E=4.3e-73 TIGR03682: diphthamide biosynthesis enzyme Dph2" amino acids 17 to 303 (287 residues), 303.9 bits, see alignment E=1.2e-94 PF01866: Diphthamide_syn" amino acids 26 to 295 (270 residues), 174.9 bits, see alignment E=1.3e-55

Best Hits

Swiss-Prot: 54% identical to DPH2_METJA: 2-(3-amino-3-carboxypropyl)histidine synthase (dph2) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

KEGG orthology group: K07561, diphthamide synthase subunit DPH2 (inferred from 100% identity to mmp:MMP0188)

Predicted SEED Role

"diptheria toxin resistance protein required for diphthamide biosynthesis (Saccharomyces)-like 1"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6M0T1 at UniProt or InterPro

Protein Sequence (309 amino acids)

>MMP_RS01045 diphthamide biosynthesis enzyme Dph2 (Methanococcus maripaludis S2)
MWDLETDRVLNEIKTRNARKVLFQAPEGLKRAVEKEIEFLKTKIDAELMIWGETCFGACD
LCDEEVTILGIDLIIHYGHEELSYVHSEIPVVFVHAYFKENEYFLKDVENILEKMENPTV
TTTIQFKKALSKFNPVVILGCRAPVDNAENILFVGTGRFHPLMMAYKLKKTVKIYNPITR
EISEISDQEIKKLIKLRIGRVSKLLLNPPKKIGVVLSTKKGQCRLKSFENVIELLKKNNI
EYIPIILNNLSQSYLFYKVDAYVICACPRIVMDDYQNYESTLLTPEELRMYLSNDFEYKF
DEILENNFY