Protein Info for MMP_RS01035 in Methanococcus maripaludis S2

Annotation: threonylcarbamoyl-AMP synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 206 TIGR00057: tRNA threonylcarbamoyl adenosine modification protein, Sua5/YciO/YrdC/YwlC family" amino acids 14 to 201 (188 residues), 198.5 bits, see alignment E=3.4e-63 PF01300: Sua5_yciO_yrdC" amino acids 24 to 193 (170 residues), 168.5 bits, see alignment E=5.4e-54

Best Hits

Swiss-Prot: 54% identical to TSAC_METJA: Putative threonylcarbamoyl-AMP synthase (MJ0062) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

KEGG orthology group: K07566, putative translation factor (inferred from 100% identity to mmp:MMP0186)

Predicted SEED Role

"TsaC protein (YrdC domain) required for threonylcarbamoyladenosine t(6)A37 modification in tRNA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6M0T2 at UniProt or InterPro

Protein Sequence (206 amino acids)

>MMP_RS01035 threonylcarbamoyl-AMP synthase (Methanococcus maripaludis S2)
MKTFEISESNLKNCEKEIETAKKMILGGKIILCGTDTLYGLSTNALDKNAIEKIYSIKER
DPNKPISISLGEKKQIENYVYVDETAKKIIEKFMPGPITVILKKKDIIPDILGKDDVGVR
IPDNDVIRKIAVVPLTTTSANISGKTAPTSLEEVDQIIKEKVDLIVDTGPCKYKVQSTIV
KVIDGKIELIREGKIPFEEILKAVNE