Protein Info for MMP_RS00845 in Methanococcus maripaludis S2

Annotation: acetate--CoA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02188: acetate--CoA ligase
  • amino acids 10 to 628 (619 residues), 885.2 bits, E=1.1e-270
• PF16177: ACAS_N
  • amino acids 13 to 66 (54 residues), 77.7 bits, 6.9e-26
• PF00501: AMP-binding
  • amino acids 74 to 461 (388 residues), 300.8 bits, E=2.1e-93
• PF13193: AMP-binding_C
  • amino acids 516 to 596 (81 residues), 78.9 bits, E=6.6e-26

Best Hits

Swiss-Prot: 58% identical to ACSA_PYRAE: Acetyl-coenzyme A synthetase (acsA) from Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)

KEGG orthology group: K01895, acetyl-CoA synthetase [EC: 6.2.1.1] (inferred from 100% identity to mmp:MMP0148)

MetaCyc: 47% identical to 3-hydroxypropionyl-CoA synthetase subunit (Metallosphaera sedula)
RXN-9086 [EC: 6.2.1.36]

Predicted SEED Role

"Acetyl-coenzyme A synthetase (EC 6.2.1.1)" in subsystem Ketoisovalerate oxidoreductase or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 6.2.1.1)

MetaCyc Pathways

• L-isoleucine biosynthesis V (3/3 steps found)
• acetate and ATP formation from acetyl-CoA III (1/1 steps found)
• acetate conversion to acetyl-CoA (1/1 steps found)
• ethanol degradation II (2/3 steps found)
• ethanol degradation III (1/3 steps found)
• ethanol degradation IV (1/3 steps found)
• superpathway of acetate utilization and formation (1/3 steps found)
• chitin deacetylation (1/4 steps found)
• adlupulone and adhumulone biosynthesis (1/6 steps found)
• colupulone and cohumulone biosynthesis (1/6 steps found)
• lupulone and humulone biosynthesis (1/6 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (2/9 steps found)
• cis-geranyl-CoA degradation (1/9 steps found)
• 3-hydroxypropanoate cycle (3/13 steps found)
• glyoxylate assimilation (3/13 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (4/18 steps found)
• superpathway of bitter acids biosynthesis (3/18 steps found)
• superpathway of the 3-hydroxypropanoate cycle (3/18 steps found)

KEGG Metabolic Maps

• Glycolysis / Gluconeogenesis
• Propanoate metabolism
• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.1

Use Curated BLAST to search for 6.2.1.1 or 6.2.1.36

Sequence Analysis Tools

See Q6M0X0 at UniProt or InterPro

Protein Sequence (636 amino acids)

>MMP_RS00845 acetate--CoA ligase (Methanococcus maripaludis S2)
MKTGDKYIMLEKYSELQQKALDDPEKFWGEQAKCLEWDKTWDKALDWNPPYAKWFKGGSL
NASYNCVDRHIKGNRRNKAAIICEKEDGSCGILTYYELYREVNKFANVLENLGVEKGDVV
TIYMPMMTEAIIAMLACSRIGAIHNVVFSGFSSDALADRINDSKSKILVTTNLLYRRGKE
IDLKKILDKALLNCNSIKHVVYAKRGIEEFNLVSGKEYFWDELMGGAKSYVEPVPVESEH
PLFILYTSGTTGSPKGVVHSTGGYLTYATKTMDWTWGLNEMDVFWCTADIGWITGHTYVV
YGPLSLGATIVLYEGAIDYPEPDRLWGIVENHGVTILYTAPTAIRMLMMYGEKWVNTHDL
STLRLLGSVGEPINPRAWKWYYKVIGKEKCPICDCYWQTETGGHIIYPPIGIQSVPLKPG
SATFPGIGIDVDVVDNEGNPVDANVKGNLIIKRPWPGMLAGLWNNDERYRAAYWDRFKNK
FSTSDYAIKDQDGYIWVLGRSDEVLNVSGHRIGTAELEHELVSNKMVAESAVVGKPDDVK
GEVPVAFVILNEKYRELPSNEVKAELIKHIRDTVGPIGTPAMIFFVNKLPKTRSGKIMRR
ILKKLIMGEEIGDVTTLEDNTSLEEVKKELEDFKYD