Protein Info for MMP_RS00840 in Methanococcus maripaludis S2

Annotation: nitrogenase iron protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 292 PF02374: ArsA_ATPase" amino acids 2 to 44 (43 residues), 39 bits, see alignment 1.5e-13 PF00142: Fer4_NifH" amino acids 2 to 277 (276 residues), 337.3 bits, see alignment E=1.9e-104 TIGR01287: nitrogenase iron protein" amino acids 2 to 284 (283 residues), 412.4 bits, see alignment E=4.3e-128 PF13614: AAA_31" amino acids 4 to 85 (82 residues), 45.2 bits, see alignment E=2.7e-15 PF01656: CbiA" amino acids 6 to 235 (230 residues), 46.8 bits, see alignment E=7e-16

Best Hits

Swiss-Prot: 77% identical to NIFH2_METTL: Nitrogenase iron protein 2 (nifH2) from Methanothermococcus thermolithotrophicus

KEGG orthology group: K02588, nitrogenase iron protein NifH [EC: 1.18.6.1] (inferred from 100% identity to mmp:MMP0147)

MetaCyc: 42% identical to ni-sirohydrochlorin a,c-diamide reductive cyclase CfbC subunit (Methanosarcina acetivorans C2A)
RXN-8061 [EC: 6.3.3.7]

Predicted SEED Role

"Nitrogenase subunit NifH paralog, type 1"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.18.6.1

Use Curated BLAST to search for 1.18.6.1 or 6.3.3.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6M0X1 at UniProt or InterPro

Protein Sequence (292 amino acids)

>MMP_RS00840 nitrogenase iron protein (Methanococcus maripaludis S2)
LKQIAFYGKGGIGKSTTVCNLAAALSKSGKKVIVVGCDPKHDCTSNLRSGEDIPTVLDVL
REKGIDKLGIETIIRENLLKKEDIIYEGFNGIYCVEAGGPKPGYGCAGRGVIVVIDLLKK
MNVFEDLDVDVVLYDVLGDVVCGGFAMPLRMGLADQIYVVTSSDYMALYAANNICSGISQ
FVKRGGSTLGGIIYNVRGSMDAFDIVSEFANQLNANIIGKVPNSPIINEAEIDGQTAIEY
APEEEISKIYMELAESIYQNNTGTMPNPLENAQIMQIGKMIKERIKKQKITE