Protein Info for MMP_RS00570 in Methanococcus maripaludis S2

Annotation: protein-L-isoaspartate O-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 212 PF01135: PCMT" amino acids 9 to 209 (201 residues), 251.6 bits, see alignment E=1.7e-78 TIGR00080: protein-L-isoaspartate O-methyltransferase" amino acids 9 to 210 (202 residues), 262.9 bits, see alignment E=1.2e-82 PF00398: RrnaAD" amino acids 70 to 139 (70 residues), 29.3 bits, see alignment E=1.2e-10 PF13847: Methyltransf_31" amino acids 75 to 149 (75 residues), 36 bits, see alignment E=1.3e-12 PF01209: Ubie_methyltran" amino acids 75 to 152 (78 residues), 27.4 bits, see alignment E=5.3e-10 PF13649: Methyltransf_25" amino acids 80 to 152 (73 residues), 28.8 bits, see alignment E=4.2e-10

Best Hits

Swiss-Prot: 100% identical to PIMT_METMP: Protein-L-isoaspartate O-methyltransferase (pcm) from Methanococcus maripaludis (strain S2 / LL)

KEGG orthology group: K00573, protein-L-isoaspartate(D-aspartate) O-methyltransferase [EC: 2.1.1.77] (inferred from 100% identity to mmp:MMP0102)

MetaCyc: 40% identical to protein-L-isoaspartate O-methyltransferase (Escherichia coli K-12 substr. MG1655)
Protein-L-isoaspartate(D-aspartate) O-methyltransferase. [EC: 2.1.1.77]

Predicted SEED Role

"Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77)" in subsystem Ton and Tol transport systems (EC 2.1.1.77)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.77

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6M116 at UniProt or InterPro

Protein Sequence (212 amino acids)

>MMP_RS00570 protein-L-isoaspartate O-methyltransferase (Methanococcus maripaludis S2)
MPLNEIIPVIENLISRGYIKKQSVIDAILSVPRHKFISKSMESYAYVDSPLEIGYGQTIS
AIHMVGIMCEELDLDEGQNVLEVGTGSGYHAAVVSKIVGESGKVTTIERIPELFENSKKT
LSELGYNNVEVVLGDGTKGYLENAPYDRIYVTASGPDVPKALFKQLNDGGILLAPVGAHF
QTLMRYTKINGSISEEKLLEVAFVPLIGENGF