Protein Info for MMP_RS00495 in Methanococcus maripaludis S2

Annotation: hydroxymethylglutaryl-CoA reductase (NADPH)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 408 TIGR00533: hydroxymethylglutaryl-CoA reductase (NADPH)" amino acids 8 to 408 (401 residues), 631.7 bits, see alignment E=2.5e-194 PF00368: HMG-CoA_red" amino acids 34 to 408 (375 residues), 455.8 bits, see alignment E=5.8e-141

Best Hits

Swiss-Prot: 75% identical to HMDH_METJA: 3-hydroxy-3-methylglutaryl-coenzyme A reductase (hmgA) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

KEGG orthology group: K00021, hydroxymethylglutaryl-CoA reductase (NADPH) [EC: 1.1.1.34] (inferred from 100% identity to mmp:MMP0087)

MetaCyc: 80% identical to hydroxymethylglutaryl-CoA reductase monomer (Methanothermococcus thermolithotrophicus)
Hydroxymethylglutaryl-CoA reductase (NADPH). [EC: 1.1.1.34]

Predicted SEED Role

"Hydroxymethylglutaryl-CoA reductase (EC 1.1.1.34)" in subsystem Archaeal lipids or Isoprenoid Biosynthesis or Leucine Degradation and HMG-CoA Metabolism (EC 1.1.1.34)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.34

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6M131 at UniProt or InterPro

Protein Sequence (408 amino acids)

>MMP_RS00495 hydroxymethylglutaryl-CoA reductase (NADPH) (Methanococcus maripaludis S2)
MENNVNIDEIVEKLLKKEIKVYQLDSKFGERNAVIARRKYVEKLSNVETRHIQEYTLDEK
LAMQKNIENMIGAVQIPLGFAGPISINGKYAQGEFNVPLATTEGALVASINRGCSIITKC
GGATVRVIDDKMTRAPIIKTNSVVDALKLKEWILDNFAKIKEIAESTTRHGKLIQISPIL
IVGRNVYPRFTFKTGDAMGMNMVTIATEKACSFIESELKKEGIIIDTVALSGNVCVDKKP
AAINLIEGRGKSVVAEVFLKEEYVEKYLKTTSKAIEQVNTYKNLIGSAISSSLGFNAQYA
NIVGALFLATGQDEAHIVEGSMGITTAECTGDGLYFSVTLPDLPVATIGGGTRVETQREC
LEILGCAGAEKAVKFAEIAGAAVLAGELSLIGALAAGHLAKAHSELGR