Protein Info for MMP_RS00160 in Methanococcus maripaludis S2

Annotation: DNA polymerase II large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1131 TIGR00354: DNA polymerase II, large subunit DP2" amino acids 10 to 1129 (1120 residues), 1800.6 bits, see alignment E=0 PF03833: PolC_DP2_N" amino acids 12 to 281 (270 residues), 402.4 bits, see alignment E=1.2e-124 PF24844: PolC_DP2_central" amino acids 309 to 686 (378 residues), 419.6 bits, see alignment E=1.6e-129 PF24846: PolC_DP2_cat" amino acids 710 to 1004 (295 residues), 472.1 bits, see alignment E=8.9e-146

Best Hits

Swiss-Prot: 100% identical to DP2L_METMP: DNA polymerase II large subunit (polC) from Methanococcus maripaludis (strain S2 / LL)

KEGG orthology group: K02322, DNA polymerase II large subunit [EC: 2.7.7.7] (inferred from 100% identity to mmp:MMP0026)

Predicted SEED Role

"Archaeal DNA polymerase II large subunit (EC 2.7.7.7)" in subsystem DNA replication, archaeal (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6M191 at UniProt or InterPro

Protein Sequence (1131 amino acids)

>MMP_RS00160 DNA polymerase II large subunit (Methanococcus maripaludis S2)
MLHVSASKGMTEYFKNILDDVSNLYNLAEECRKNGYDVTDHVEIPLAKDMADRVEGIVGP
KNVAERIRELVSEFGKEPAALEIAKEIVEGKFGEFGREVGAEQAVRTALAVITEGIVAAP
LEGIAYVKIKKNSDNSEYLAIYFAGPIRSAGGTAQALAVLVGDYVRKNMGLDRFKPTEDE
VERYGEEVDLYQSEVTTFQYQPKAEEIRVAVRNISVEITGEATDDVEVSGHRDLPRVETN
QIRGGALLALVEGVLLKAPKILRHVDKLNIEGWNWLKELKSKKEEVIEELEEENDEYNYE
DEEDLSQYEDYEVEAVTKFIGEVIAGRPVFSHPSKKGGFRLRYGRSRNTGFATDGFHPAI
MYLVDDFMAVGTQLKTERPGKATCVVPVDSIEGPIVKLNDGSVLKIDTVEKAKQYTDEVQ
EILFLGDILVNYGDFLENNHTVLPSSWCTEWYEKILKSQNLEYTEEFIKNPGQKEAVNYA
KITKTPLHPKYTYFWHDISKENISTLRSWVIGGKYNPSNDSWELNYDPEDEEISNAKRYL
ELIGCPHIVMEEKVEIFEYYPFLYSLGYDFDEKRDMIDNIDEKLQNTKNNMHFINTIAPF
EIRRNAYIYVGARMGRPEKAAARKMKPPVNGLFPIGNAGALVRLINKAVDEGKTDEIEIA
NVKCSCGKVSLYRTCPFCGNSVEPTGPSRIKLPIKDYWYKTLENLKINKPGDVKCIKGMT
SKDKIIEPLEKAILRAKNNIYVFKDGTTRFDCTDVPVTHFKPVEIHVPIEKLKSLGYLKD
IHGNPLENEDQVLELKVQDVIVPESCMDYFLNVSGFIDDLLEKYYKKDRFYNVNTREDLV
GHLIIGMAPHTSAGMVGRIIGYSNANVGYAHPYFHASKRRNCDGDEDAFFLLLDAFMNFS
KRFLPDKRGGQMDAPLVLTTILDPKEVDGEVHNMDSMWEYPLEFYEKSLEGIAPKEIKKM
METIEDRLDKDSQYEGIGYTHETSKIDEGPPICAYKTLGSMMEKTSAQLAVAKKIRATDE
RDVAEKVIQSHFVPDLIGNLRAFSRQGVRCKCGAKYRRMPLKGVCRKCGSRLILTVSKGA
VEKYMNVSQTMAEKYDASDYIKQRLEIIKSGIDSLFVNDKRKQVKIEDFFK