Protein Info for MMP_RS00070 in Methanococcus maripaludis S2

Annotation: DNA-directed DNA polymerase II small subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 585 PF00149: Metallophos" amino acids 320 to 526 (207 residues), 45.4 bits, see alignment E=1.9e-15 PF04042: DNA_pol_E_B" amino acids 393 to 522 (130 residues), 44.8 bits, see alignment E=1.5e-15

Best Hits

KEGG orthology group: K02323, DNA polymerase II small subunit [EC: 2.7.7.7] (inferred from 100% identity to mmp:MMP0008)

Predicted SEED Role

"Archaeal DNA polymerase II small subunit (EC 2.7.7.7)" in subsystem DNA replication, archaeal (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6M1A8 at UniProt or InterPro

Protein Sequence (585 amino acids)

>MMP_RS00070 DNA-directed DNA polymerase II small subunit (Methanococcus maripaludis S2)
MINDFLNIQVLLAPESYSRLQNLDSNDFSDLFNKIKEFKSKKDDFILLDSHFLNIFLGNN
LKNIFEEYGKFDFLAYYTSNEVASEEKIIEKDDFEPVNEVKLENEEIIEPKRENKVYDLD
FEDIEEEEPELDEIHKKLEEERKERILEVRSIRESVNNKINYYAKDIDAQIHVYDEYDVT
GKSTCEGTLDDFVKYFKDRCSTLRKIIELKNQKKAYPLNQLFRRKKENEVFIAGIVSDVN
STKNGHKMVELEDENGTFRVLLMKDKMEKGDLPSDVLLDEVIGFEGTINDKGDLMFVDRA
FRPDITPKPTKTTDEKIYTAFLSDVHVGSREFLNKVFAKFIRFLNGEVNNGLEEKIVSRL
KYISIAGDLVDGVGIYPGQEYDLYDVDIISQYAEVAAYLEQVPEHIKVIICPGNHDALRP
AEPQPTFDESITSLFPKENVTFVGNPGVVNMHGLDLLMYHGRSFDDIIGQISSAQYTNPP
SIMTELLKRRHLCPTYGGRCPIAPEHVDYLAIHTEPDIFHTGHIHINGYDNYHGVRMINS
GTFQEQTDFQKRMGIKPTPGIIPIQDLSKREQHMIEWNQGKIEIN