Protein Info for MMJJ_RS08800 in Methanococcus maripaludis JJ

Annotation: sugar phosphate nucleotidyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 411 TIGR03992: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase" amino acids 1 to 388 (388 residues), 527.1 bits, see alignment E=1.3e-162 PF00483: NTP_transferase" amino acids 3 to 224 (222 residues), 155 bits, see alignment E=4.2e-49 PF12804: NTP_transf_3" amino acids 3 to 109 (107 residues), 45.6 bits, see alignment E=1.3e-15 PF00132: Hexapep" amino acids 355 to 389 (35 residues), 31.6 bits, see alignment 1.4e-11

Best Hits

Swiss-Prot: 96% identical to GLMU_METMP: Bifunctional protein GlmU (MMP1076) from Methanococcus maripaludis (strain S2 / LL)

KEGG orthology group: K04042, bifunctional UDP-N-acetylglucosamine pyrophosphorylase / Glucosamine-1-phosphate N-acetyltransferase [EC: 2.3.1.157 2.7.7.23] (inferred from 96% identity to mmp:MMP1076)

MetaCyc: 47% identical to multifunctional glucose-1-phosphate thymidylyltransferase/hexosamine-1-phosphate N-acetyltransferase/UDP-N-acetylhexosamine diphosphorylase (Sulfurisphaera tokodaii)
RXN-13760 [EC: 2.7.7.83]; Glucose-1-phosphate thymidylyltransferase. [EC: 2.7.7.83, 2.7.7.24]; UDP-N-acetylglucosamine diphosphorylase. [EC: 2.7.7.83, 2.7.7.24, 2.7.7.23]; 2.7.7.- [EC: 2.7.7.83, 2.7.7.24, 2.7.7.23]; RXN-19880 [EC: 2.7.7.83, 2.7.7.24, 2.7.7.23, 2.3.1.276]; Glucosamine-1-phosphate N-acetyltransferase. [EC: 2.7.7.83, 2.7.7.24, 2.7.7.23, 2.3.1.276, 2.3.1.157]

Predicted SEED Role

"N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)" in subsystem Peptidoglycan Biosynthesis or Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.3.1.157, EC 2.7.7.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.157, 2.7.7.23

Use Curated BLAST to search for 2.3.1.157 or 2.3.1.276 or 2.7.7.23 or 2.7.7.24 or 2.7.7.83

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (411 amino acids)

>MMJJ_RS08800 sugar phosphate nucleotidyltransferase (Methanococcus maripaludis JJ)
MDAIILCAGKGTRLHPITESRPKPMIPIAGKPILEHIIEKIENHVEKIYLIVGYQKEKII
EYFKDNPKIEYILQEKQLGTGHAVLTAKNFIKNDFLVLNGDVIFEDSIDEILTYENAVSL
SKVDNPENFGVIELGYDNKVINLLEKPKKEEITSNLINAGIYKLQNSVFGILENLVPSER
GEIELTDALKTLVENGKLHGVELKGYWNDIGHPWDVLSANNHFLNKIISKVSGKIEDNVS
ITGNVMIEEGAVIKSNSVIEGPVIIKSGSIVGPLAYIRPNTVLMNNTFVGNSSEIKGSII
FENTKIPHLSYVGDSIIGANCNFGCNTITANLRFDDKPVIVNIKGKPVKSVRKLGAIIGD
CVKTGIQVSFMPGVKIGSNSLIGANCLIDRDIDQDSFVYKKDELVITKKRN