Protein Info for MMJJ_RS05950 in Methanococcus maripaludis JJ

Annotation: bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08543: Phos_pyr_kin
  • amino acids 10 to 233 (224 residues), 176.1 bits, E=8.5e-56
• PF10120: ThiN
  • amino acids 281 to 391 (111 residues), 40.1 bits, E=3.1e-14

Best Hits

Swiss-Prot: 47% identical to THIDN_METJA: Bifunctional thiamine biosynthesis protein ThiDN (thiDN) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

KEGG orthology group: K00941, hydroxymethylpyrimidine/phosphomethylpyrimidine kinase [EC: 2.7.1.49 2.7.4.7] (inferred from 93% identity to mmp:MMP1639)

Predicted SEED Role

"Phosphomethylpyrimidine kinase (EC 2.7.4.7) / Thiamin-phosphate synthase ThiN (EC 2.5.1.3)" in subsystem Thiamin biosynthesis (EC 2.5.1.3, EC 2.7.4.7)

MetaCyc Pathways

• thiamine diphosphate salvage II (5/5 steps found)
• thiamine diphosphate salvage V (3/3 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I (2/2 steps found)
• hydroxymethylpyrimidine salvage (2/2 steps found)
• thiamine diphosphate biosynthesis I (E. coli) (2/2 steps found)
• thiamine diphosphate biosynthesis II (Bacillus) (2/2 steps found)
• thiamine diphosphate salvage IV (yeast) (4/7 steps found)
• thiamine diphosphate biosynthesis III (Staphylococcus) (1/3 steps found)
• thiamine diphosphate biosynthesis IV (eukaryotes) (1/3 steps found)
• thiamine diphosphate formation from pyrithiamine and oxythiamine (yeast) (4/8 steps found)
• superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (3/7 steps found)
• superpathway of thiamine diphosphate biosynthesis I (5/10 steps found)
• superpathway of thiamine diphosphate biosynthesis II (5/11 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis II (1/8 steps found)

KEGG Metabolic Maps

• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.3

Use Curated BLAST to search for 2.5.1.3 or 2.7.1.49 or 2.7.4.7

Protein Sequence (400 amino acids)

>MMJJ_RS05950 bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase (Methanococcus maripaludis JJ)
MNILTVGGFDPTGGAGIVADVKTIKELQKNPLSIITSIIPQNNNKVFLKKDLSKEEIKAQ
FDAIFEDFDVNWVKTGVLTIDSIDIILEFKEKYGFNIICDPVLKSTTDFEFSDDFLNKKY
FEFFKECFLITPNLKEFEIIEKMFETSKSNLNDINVLVTGKTDVLKINNKNIEITGKYVE
KEVHGTGCTYSSAITCFLSTGMTVKDSIVSAKEFVLGSVIYAEKTKFGYNSNPTSIDKES
VEKNLAYAFNLLKNIDISLADPNFIISESILLPETYLDIATVKNNGDINFGISNTNSDLL
LKLKEFNPKIRSCISLKSDESIKSKLENSKLSICPMKLLNDPISSLKEELIQNRSRTLFD
ENFSDILYFEEKKPNLTILGDNSLEIVKKLEKIEKLIKNR