Protein Info for MMJJ_RS03735 in Methanococcus maripaludis JJ

Annotation: 2-oxoacid:acceptor oxidoreductase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03710: 2-oxoacid:acceptor oxidoreductase, alpha subunit
  • amino acids 6 to 564 (559 residues), 658.3 bits, E=4.9e-202
• PF01558: POR
  • amino acids 15 to 176 (162 residues), 108.4 bits, E=4.7e-35
• PF01855: POR_N
  • amino acids 212 to 442 (231 residues), 178.7 bits, E=1.6e-56

Best Hits

KEGG orthology group: K00174, 2-oxoglutarate ferredoxin oxidoreductase subunit alpha [EC: 1.2.7.3] (inferred from 96% identity to mmp:MMP0306)

Predicted SEED Role

"2-oxoglutarate oxidoreductase, alpha subunit (EC 1.2.7.3)" (EC 1.2.7.3)

MetaCyc Pathways

• incomplete reductive TCA cycle (6/7 steps found)
• reductive TCA cycle I (6/11 steps found)
• TCA cycle V (2-oxoglutarate synthase) (4/9 steps found)
• reductive TCA cycle II (5/12 steps found)
• TCA cycle VI (Helicobacter) (2/9 steps found)
• TCA cycle VII (acetate-producers) (2/9 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (34/56 steps found)

KEGG Metabolic Maps

• Citrate cycle (TCA cycle)
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 1.2.7.3

Use Curated BLAST to search for 1.2.7.3

Protein Sequence (570 amino acids)

>MMJJ_RS03735 2-oxoacid:acceptor oxidoreductase subunit alpha (Methanococcus maripaludis JJ)
MNLEKEVSIVLGGAAGQGIQTIEESLTKILKLSGYNVFATKEYMSRVRGGINTTEIVISS
EKQRSFLKRIDLLVTFKKGVLNHLKDRISENTIILGEKENIDEEFINNKNIIDIPIQEIS
KNIGNVLFSNTVGSGIILGIFDCDLELFNKYLEDKFSKKGKEIVDKNIEAAEKGYELGKK
ILKDLDLSLNIKKNEKINNEIILSGTEAVALGAAKAGCNFVSFYPMTPSTGVSTFLATHS
KDLGIIVEQVEDEISAINMAIGAGYAGARSLVTTSGGGFSLMCEAVSLSGMTETPVVIYL
AQRPGPSTGLPTRTAQGDLELALYSGHGEFPKIIYAPGKIEEAHKISQIAFNMADKYQVP
VFVLSDEYLADTYYNLSDNELETLKKENNVVKTSLDYKRYELNENIVSKRGIPEYGEGIV
LACGNEHDEFGDITEDIDLINKMTEKRNKKLDLIKKEAIEPEFIGNETYKNLIVSWGSTF
YLIKDALEKLNLKDTAFLHFNQVYPISDSAEKYLKNAEKVINIELNYTGQLGKLLKREFG
IEMNDSILKYDGRVFAVEEIIEKIEKVLRE