Protein Info for LRK55_RS17830 in Rhodanobacter denitrificans MT42

Annotation: molecular chaperone DnaK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 639 TIGR02350: chaperone protein DnaK" amino acids 3 to 602 (600 residues), 948.6 bits, see alignment E=6.4e-290 PF00012: HSP70" amino acids 4 to 601 (598 residues), 881.7 bits, see alignment E=2.2e-269 PF06723: MreB_Mbl" amino acids 115 to 377 (263 residues), 42.4 bits, see alignment E=4.3e-15

Best Hits

Swiss-Prot: 84% identical to DNAK_XANOP: Chaperone protein DnaK (dnaK) from Xanthomonas oryzae pv. oryzae (strain PXO99A)

KEGG orthology group: K04043, molecular chaperone DnaK (inferred from 84% identity to xom:XOO_1913)

MetaCyc: 74% identical to chaperone protein DnaK (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Chaperone protein DnaK" in subsystem Heat shock dnaK gene cluster extended or Protein chaperones

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (639 amino acids)

>LRK55_RS17830 molecular chaperone DnaK (Rhodanobacter denitrificans MT42)
MGKIIGIDLGTTNSCVSVMDGSTTKVIENSEGDRTTPSIVAFTKDGEVLVGASAKRQSVT
NPKNTFYAVKRLIGRKFTDKEVQKDLHIVPYGILAHDNGDAWVQTSDGKKMAPQEIAAKV
LMKMKKTAEDYLGETITEAVITVPAYFNDSQRQATKDAGKIAGLDVKRIINEPTAAALAY
GLDKKGGDRKIAVYDLGGGTFDVSIIEIAEVDGEKQFEVLATNGDTFLGGEDFDMRVIDY
LVEEFQKEQGIDLRKDPLALQRLKDAAERAKIELSSNHQTDVNLPYVTADASGPKHLNIK
LTRAKLEALVEDLVKRTIEPCRTALNDAGLRVADIDDVILVGGQTRMPKVQEAVKDFFGK
EPRKDVNPDEAVAVGAAIQGGVLGGSVKDVLLLDVTPLSLGIETMGGVMTKLIEKNTTVP
TKASQTFSTADDNQTAVTVHVLQGERERASANKSLGKFDLQGIEPAPRGMPQIEVTFDID
ANGILHVSAKDKKTGKEQKIEIKAGSGLSEEEIQRMVADAEANKEEDKKFHELVATRNKA
DQLVHATRSALKEHGGKVPADQLSSIEGALSDLEKVKDGDDKAAIEAKVEKLEQVAQALH
AAAQGGGTADAGAQSAPGGSAQPDDVVDAEFTEVKDDKK