Protein Info for LRK55_RS15745 in Rhodanobacter denitrificans MT42

Annotation: UDP-N-acetylmuramate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 51% identical to MURB_PSEAE: UDP-N-acetylenolpyruvoylglucosamine reductase (murB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00075, UDP-N-acetylmuramate dehydrogenase [EC: 1.1.1.158] (inferred from 54% identity to smt:Smal_1723)

Predicted SEED Role

"UDP-N-acetylenolpyruvoylglucosamine reductase (EC 1.1.1.158)" in subsystem Peptidoglycan Biosynthesis or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 1.1.1.158)

MetaCyc Pathways

peptidoglycan biosynthesis I (meso-diaminopimelate containing) (10/12 steps found)
UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (7/8 steps found)
UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (6/8 steps found)
UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
peptidoglycan biosynthesis III (mycobacteria) (10/15 steps found)
peptidoglycan recycling I (9/14 steps found)
peptidoglycan biosynthesis II (staphylococci) (11/17 steps found)
peptidoglycan biosynthesis IV (Enterococcus faecium) (11/17 steps found)
peptidoglycan biosynthesis V (β-lactam resistance) (10/17 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.158

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (342 amino acids)

>LRK55_RS15745 UDP-N-acetylmuramate dehydrogenase (Rhodanobacter denitrificans MT42)
MGGYALIENASLAARNTLRVDARARLLAEIRDAAKLPELLDFPAVRSGRLLVLGEGSNVL
FTGDFDGTVLAMATRGVQVESDGDGARIAVAAGERWDDFVRWTLGQGYAGLENLILIPGT
VGAAPIQNIGAYGCEVAEFIDSVEAWDTHERQVATLDRAACAFAYRDSLFKHEPGRYVVT
AVRFVLPRSRPLRTDYAGIDEELARMGVDRPAPFHVAEAVMRLRLRKLPDPAVIGNAGSF
FKNPIVDAALANALQREHPGLAAWPQPDGRSKLSAAWLIEKAGFKGLREGDAGISNRHAL
VLVNHGHATGPQLWALAQQVMHGVRERFGVQLEPEPVVIGAR