Protein Info for LRK55_RS10875 in Rhodanobacter denitrificans MT42
Annotation: NADP-dependent malic enzyme
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 59% identical to MAO2_HAEIN: NADP-dependent malic enzyme (maeB) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
KEGG orthology group: K00029, malate dehydrogenase (oxaloacetate-decarboxylating)(NADP+) [EC: 1.1.1.40] (inferred from 72% identity to xca:xccb100_0853)Predicted SEED Role
"NADP-dependent malic enzyme (EC 1.1.1.40)" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP (EC 1.1.1.40)
MetaCyc Pathways
- gluconeogenesis I (11/13 steps found)
- C4 photosynthetic carbon assimilation cycle, NADP-ME type (4/7 steps found)
- C4 photosynthetic carbon assimilation cycle, PEPCK type (8/14 steps found)
- methylgallate degradation (2/6 steps found)
- protocatechuate degradation I (meta-cleavage pathway) (3/8 steps found)
- superpathway of vanillin and vanillate degradation (3/10 steps found)
- syringate degradation (3/12 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of terpenoids and steroids
- Carbon fixation in photosynthetic organisms
- Pyruvate metabolism
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.1.1.40
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (763 amino acids)
>LRK55_RS10875 NADP-dependent malic enzyme (Rhodanobacter denitrificans MT42) MSLPEELRLAALEYHRLPRPGKIKVTPTTSLLTQRDLSLAYSPGVAAACDAIVEDPTSAA EYTARSNLVAVISNGTAVLGLGNIGPLAAKPVMEGKGVLFQKFAGIDVFDIEIDENDPDK LVDIIASLEPTFGGINLEDIKAPECFIVERKLRERMKIPVFHDDQHGTSIIVGAAVINAL VVVGKKIEDIRVATTGAGAAGIACLDMLVALGVKPEHILAFDRHGVIHTERTDLDPDKQR YARDTGARTLAEIVEGADVFLGLSAGGILKPEMVATMAARPVIFALANPNPEITPEDAKA VRPDCIMATGRSDYPNQVNNALCFPYLFRGALDVGATVINEAMKIACVKAIAALARRESS DVSARAYGGKPPSFGADYLIPQPFDPRLLIQLPPAVARAAMESGVATRPIADFAAYNDQL TSFVFRTGLLMKPVFERAKSAPCRLVYAEGEEETVLRAVQVVVDEGLARPILIGRPEVIE KRIHRAGLRIRPGVDIEICNINFDPRFDDYWRHYHRLMERRGVTPSMAKAVVRSRPTAIA ALMVERGEADAMICGLVGQYQGKLRYIRDIIGLDAGVVEPSAMAAVSTDKGTFFYLDTHV QDDPCPEQIAEATLQASIRLKLFGIVPKIALLSGGNFGSRDTCGAKKMRKALELIRARAP RLEVEGEMQADAALVPALREKLFPHSRLEGKANVFVFPNLDAANIAYNMTRMLSDGVVIG PILMGVAKPAHILMPQSTVRRVVNMSAVACVEAQIRAANRHTG