Protein Info for LRK55_RS09375 in Rhodanobacter denitrificans MT42

Annotation: bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01173: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase
  • amino acids 7 to 454 (448 residues), 587 bits, E=1.3e-180
• PF01128: IspD
  • amino acids 7 to 121 (115 residues), 32.9 bits, E=1.5e-11
• PF12804: NTP_transf_3
  • amino acids 8 to 121 (114 residues), 80 bits, E=5.8e-26
• PF00483: NTP_transferase
  • amino acids 8 to 214 (207 residues), 40.8 bits, E=5.1e-14
• PF00132: Hexapep
  • amino acids 395 to 429 (35 residues), 36.2 bits, 8.1e-13
• PF14602: Hexapep_2
  • amino acids 396 to 429 (34 residues), 33 bits, 1e-11

Best Hits

Swiss-Prot: 66% identical to GLMU_XANOR: Bifunctional protein GlmU (glmU) from Xanthomonas oryzae pv. oryzae (strain KACC10331 / KXO85)

KEGG orthology group: K04042, bifunctional UDP-N-acetylglucosamine pyrophosphorylase / Glucosamine-1-phosphate N-acetyltransferase [EC: 2.3.1.157 2.7.7.23] (inferred from 66% identity to xoo:XOO0736)

MetaCyc: 51% identical to fused N-acetylglucosamine-1-phosphate uridyltransferase and glucosamine-1-phosphate acetyltransferase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylglucosamine diphosphorylase. [EC: 2.7.7.23]; Glucosamine-1-phosphate N-acetyltransferase. [EC: 2.7.7.23, 2.3.1.157]

Predicted SEED Role

"N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)" in subsystem Peptidoglycan Biosynthesis or Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.3.1.157, EC 2.7.7.23)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (6/9 steps found)
• peptidoglycan recycling I (9/14 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (7/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (13/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.157 or 2.7.7.23

Protein Sequence (456 amino acids)

>LRK55_RS09375 bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU (Rhodanobacter denitrificans MT42)
MNHASLHVVILAAGAGTRMKSSRPKVLMPLAGRPLLAHVIDTARALQPAAIHVVYGHGGD
QVRAAFADQTDLRWVLQAERLGTGHAVEQALGEIPDGARVLVLYGDVPLTRADTLRQLAA
AEGGFSLLTMRLADPCGYGRVLCDGNGHVRAVIEEKDADQEQRAVNLVNTGILVASAAAL
RVWIAGLDRDNAQGEYYLTDIFAMASEEKRPALGVECADPVEASGANDALQLAGLEAEYR
QRAARALMRNGVRLADPLRVDVRGTVASGRDVELDVDVILEGRVVLGDDVRIGAFTRLKD
VQLAAGTMVQPHCDLDGVVTHGPCAIGPFARLRPGTELEAGVHVGNFVETKKTHLGEGSK
ANHLSYLGDTVVGRGVNIGAGTITCNYDGVNKFATQIGDGAFIGSNSALVAPVTIGAQAT
IGAGSVITRDAPAGELTVARGKQQTFPGWKRPVKQE