Protein Info for LRK55_RS05460 in Rhodanobacter denitrificans MT42
Annotation: glutamine--fructose-6-phosphate transaminase (isomerizing)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 58% identical to GLMS_ECOL6: Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (glmS) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
KEGG orthology group: K00820, glucosamine--fructose-6-phosphate aminotransferase (isomerizing) [EC: 2.6.1.16] (inferred from 58% identity to maq:Maqu_3872)MetaCyc: 57% identical to L-glutamine--D-fructose-6-phosphate aminotransferase (Escherichia coli K-12 substr. MG1655)
Glutamine--fructose-6-phosphate transaminase (isomerizing). [EC: 2.6.1.16]
Predicted SEED Role
"Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16)" in subsystem Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.6.1.16)
MetaCyc Pathways
- O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
- UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
- UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
- chitin biosynthesis (6/9 steps found)
- UDP-N-acetyl-D-galactosamine biosynthesis III (2/6 steps found)
- CMP-legionaminate biosynthesis I (2/10 steps found)
- superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (7/24 steps found)
- superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (13/33 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.6.1.16
Use Curated BLAST to search for 2.6.1.16
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (609 amino acids)
>LRK55_RS05460 glutamine--fructose-6-phosphate transaminase (isomerizing) (Rhodanobacter denitrificans MT42) MCGIVAAVAQRDVAPLLIAGLKALEYRGYDSSGVAVLDRGTIRRVRAKGKVREMEALYLA DPLPGGTGIAHTRWATHGVPNEANAHPHIAGRVAIVHNGIIENYVSLRAELQAGGHVFTS ETDTEVMAALIDRHIGQGMKLREAVLATVRELEGAYAIAVISSDEPDRVVGARRGAPLLV GVGIGENFLGSDAQALIQVTNRMIYLDEDDVVEITRDSVQVYGLDGTPVERAVHESELST DAVERGEYRHYMQKEIFEQPRAVADTLEARIGPHGVLPNIFGIDSGALLQATRGLHIVAC GTSYHAGLVAKYWIEEYARLPVAVEVASEYRYRETVVPEGTLFVAISQSGETADTLAAMR ESRRRGYLGTLAICNVPESSVVREADLKLMTRAGPEIGVASTKAFTTQLAGLALLTLELA RRHGLDDDRYAALCAELQRLPRAIGEALQMEPAIIELAADFIQRQHALFLGRGVQYPVAL EGALKLKEISYIHAEAYAAGELKHGPLALVDENMPVVAVAPNGPLLDKLKSNLQEVRARG GRLIVFADGAAGMDDMAGSGTMLRVESGGSFIAPAVFTVPLQLLAYHVAVLRGTDVDQPR NLAKSVTVE