Protein Info for LRK55_RS02030 in Rhodanobacter denitrificans MT42

Annotation: enoyl-CoA hydratase-related protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00378: ECH_1
  • amino acids 11 to 262 (252 residues), 172.3 bits, E=1.2e-54
• PF16113: ECH_2
  • amino acids 14 to 198 (185 residues), 106.5 bits, E=2.1e-34

Best Hits

KEGG orthology group: K13766, methylglutaconyl-CoA hydratase [EC: 4.2.1.18] (inferred from 65% identity to psu:Psesu_1646)

MetaCyc: 45% identical to 3-methylglutaconyl-CoA hydratase (Pseudomonas putida)
Methylglutaconyl-CoA hydratase. [EC: 4.2.1.18]

Predicted SEED Role

"Methylglutaconyl-CoA hydratase (EC 4.2.1.18)" in subsystem Benzoate transport and degradation cluster or HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism (EC 4.2.1.18)

MetaCyc Pathways

• L-leucine degradation I (5/6 steps found)
• ferrichrome A biosynthesis (1/5 steps found)

KEGG Metabolic Maps

• Valine, leucine and isoleucine degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.18

Protein Sequence (265 amino acids)

>LRK55_RS02030 enoyl-CoA hydratase-related protein (Rhodanobacter denitrificans MT42)
MTDSIRSERRGATAWLTLNRPQVHNAFDDDLIAALTEALAAADADPAVRCVVLTGSGSTF
SAGADLNWMRGMAAASEQENREDSLRLARLMRSLQFLSKPTIARVNGAAYGGGVGLVACC
DIAIGVDTAKFALSEVKLGLVPAVISPYVVAAIGLRQARRLFVSGEVFDAAEAQRIGLLH
AAVSGSELDEAIERQVHWLAKAGPLAQREAKQLALRTGGADLAQAERIDAANAELIARLR
VSPEGQHGLAAFLDKRAPGWVGQAT