Protein Info for LRK55_RS01365 in Rhodanobacter denitrificans MT42
Annotation: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 56% identical to HIS4_STRM5: 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA) from Stenotrophomonas maltophilia (strain R551-3)
KEGG orthology group: K01814, phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase [EC: 5.3.1.16] (inferred from 56% identity to smt:Smal_1761)Predicted SEED Role
"Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (EC 5.3.1.16)" in subsystem Histidine Biosynthesis (EC 5.3.1.16)
MetaCyc Pathways
- superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
- L-histidine biosynthesis (10/10 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of plant hormones
- Histidine metabolism
Isozymes
No predicted isozymesUse Curated BLAST to search for 5.3.1.16
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (242 amino acids)
>LRK55_RS01365 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase (Rhodanobacter denitrificans MT42) MNFDVIPAIDLRGGQVVRLKQGDYAQQTTYAADPRALARRYAEAGARWLHMVDLDGARSG SLANLAVIQAITADGMQIQAGGGVRGEDDLRRLFDAGVQRVVLGSVAIRDPELVAGWLGR YGAERLTLALDTRRLGGRWSLPSAGWTEIEARTLDELAPWYAARGARHLLCTDIDRDGML AGFNLDLYRHLVATVPSLAVQASGGVRSLDDIRAAREAGAWGVILGRALLEGRFTVAEAL AA