Protein Info for LRK54_RS13180 in Rhodanobacter denitrificans FW104-10B01

Annotation: amidotransferase 1, exosortase A system-associated

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03108: exosortase A system-associated amidotransferase 1
  • amino acids 1 to 626 (626 residues), 969.1 bits, E=1.1e-295
• TIGR01536: asparagine synthase (glutamine-hydrolyzing)
  • amino acids 2 to 560 (559 residues), 485.7 bits, E=2.1e-149
• PF13522: GATase_6
  • amino acids 32 to 161 (130 residues), 144.8 bits, E=2.3e-46
• PF13537: GATase_7
  • amino acids 47 to 167 (121 residues), 152.8 bits, E=6.1e-49
• PF00733: Asn_synthase
  • amino acids 242 to 625 (384 residues), 360.6 bits, E=2.1e-111

Best Hits

KEGG orthology group: K01953, asparagine synthase (glutamine-hydrolysing) [EC: 6.3.5.4] (inferred from 60% identity to aeh:Mlg_0138)

Predicted SEED Role

"Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4) AsnH" in subsystem Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 6.3.5.4)

MetaCyc Pathways

• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• superpathway of L-asparagine biosynthesis (2/2 steps found)
• L-asparagine biosynthesis I (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.4

Use Curated BLAST to search for 6.3.5.4

Protein Sequence (649 amino acids)

>LRK54_RS13180 amidotransferase 1, exosortase A system-associated (Rhodanobacter denitrificans FW104-10B01)
MCGLAGIVSSRHVDVDERALLAMRDAQLHRGPDEAGLYLGKGVGLGHRRLSIIDLGHGQQ
PMVDETAGLALIYNGELYNFPSLKAELEARGVVFRSRCDTEVLLRAWQQWGEACLTRLVG
MFAFAVWDMRTQRIYLARDQLGIKPLYYGFTRTFELVFASELKGLLAHPGVERRLDPQAL
EDYLALGYVPDPRSIYRGIFKLPPGHWLSWHAGEPEPVPRQYWDVPFKVAADMTADEAAA
QLHGLLDRAVAGQMIADVPLGAFLSGGVDSSAVVASMSRASQQPVRTCSIGFDHGGFDES
AYARRVAEHLHTQHFEQRVSADDYDLLDTLAAVYDEPFSDSSALPTYRVCGLARTQVTVA
LSGDGGDENFAGYRRYRLHAWESGMRARLPLALRKPLFGVLGSLYPKADWAPRPLRAKTT
LQALGRDDVEAYFHTVSTTSAALRQQLYSAGFKRELQGYNALAVFRAHARRAPTDHPLLL
AQYLDFKTWLPGDILTKVDRASMAHSLEVRVPLLDHRLVEWASSLPPGLKLRGGTAKYIL
KKTLEPDLPNDVLYRSKMGFRVPVAAWLRGPLARRARDALLAGAVAECGYFEPAVLDRLL
QQHAAGRRDHSATLWSLLMLDAFLRRMQQPAAAAPAGPTRRVATAGAQP