Protein Info for LRK54_RS12305 in Rhodanobacter denitrificans FW104-10B01

Annotation: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 262 TIGR00755: ribosomal RNA small subunit methyltransferase A" amino acids 4 to 257 (254 residues), 281.5 bits, see alignment E=2.9e-88 PF00398: RrnaAD" amino acids 5 to 259 (255 residues), 227.6 bits, see alignment E=7.7e-72

Best Hits

Swiss-Prot: 55% identical to RSMA_HALHL: Ribosomal RNA small subunit methyltransferase A (rsmA) from Halorhodospira halophila (strain DSM 244 / SL1)

KEGG orthology group: K02528, 16S rRNA (adenine1518-N6/adenine1519-N6)-dimethyltransferase [EC: 2.1.1.182] (inferred from 64% identity to xal:XALc_2764)

Predicted SEED Role

"SSU rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase (EC 2.1.1.182)" (EC 2.1.1.182)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.182

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (262 amino acids)

>LRK54_RS12305 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA (Rhodanobacter denitrificans FW104-10B01)
MNARPKKSFGQHFLHDRRYIDRIVSAIAPRAEDFVVEIGPGEGALTLPLLAVAGRLTAIE
LDTDLIPGLQARAATTGELHIIHADVLKVDFSALAHSHGVPRLRIAGNLPYYISSPILFH
CVEHAAAIQDMHFMLQKEVVERMAAEPGSKVYGRLSVMLQLACRVEPLFDVPPEAFRPPP
KVESAVVRLLPLPAHELHDAPPEHVHAVVKAAFGQRRKTLANALRQLLDADAIRRADVDP
KARAETLAPADFVRLAKIYGNP