Protein Info for LRK54_RS12240 in Rhodanobacter denitrificans FW104-10B01

Annotation: undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 357 transmembrane" amino acids 73 to 91 (19 residues), see Phobius details amino acids 98 to 116 (19 residues), see Phobius details amino acids 184 to 203 (20 residues), see Phobius details TIGR01133: undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase" amino acids 1 to 351 (351 residues), 339.6 bits, see alignment E=1e-105 PF03033: Glyco_transf_28" amino acids 7 to 144 (138 residues), 122.8 bits, see alignment E=1.1e-39 PF04101: Glyco_tran_28_C" amino acids 184 to 346 (163 residues), 131.1 bits, see alignment E=4.3e-42

Best Hits

Swiss-Prot: 60% identical to MURG_ALKEH: UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) from Alkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1)

KEGG orthology group: K02563, UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase [EC: 2.4.1.227] (inferred from 60% identity to aeh:Mlg_2193)

MetaCyc: 50% identical to N-acetylglucosaminyl transferase (Escherichia coli K-12 substr. MG1655)
acetylglucosaminyltransferase. [EC: 2.4.1.227]

Predicted SEED Role

"UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (EC 2.4.1.227)" in subsystem Peptidoglycan Biosynthesis (EC 2.4.1.227)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.227

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (357 amino acids)

>LRK54_RS12240 undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase (Rhodanobacter denitrificans FW104-10B01)
MNTQAPVLIMAGGTGGHIFPGLAVAECLRAQGVPVVWLGAVGGMETKVVPAQRIELHTVA
VGGLRGKGIRTRLLAPLMLLRALFASLAVLRQVQPRSVLSMGGYVAGPGGLAAWLLRRPL
LVHEQNRVAGFTNRKLAGLAKRVLAGFADALPRAEWVGNPVREAIAALPPPAQRMVGRDG
KPRLLVLGGSLGARALNLALPQALAQLTPAQRPEVLHQCGSRGLDEAREAYAKAGVEAQV
VAFIDDMAGTYGWADLAVCRAGALTLAELAAAGLGAVLVPFPHAVDDHQTRNAEVLMAAG
AAELMQENELDVQILAQRLALLLGDRHRLLAMAEAARTLAKPDAAQVIARACLEVAA