Protein Info for LRK54_RS11805 in Rhodanobacter denitrificans FW104-10B01

Annotation: ATP-dependent DNA helicase RecG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 704 PF17191: RecG_wedge" amino acids 26 to 182 (157 residues), 46.5 bits, see alignment E=8e-16 TIGR00643: ATP-dependent DNA helicase RecG" amino acids 38 to 674 (637 residues), 724.7 bits, see alignment E=5.7e-222 PF04851: ResIII" amino acids 282 to 442 (161 residues), 34.9 bits, see alignment E=3.7e-12 PF00270: DEAD" amino acids 286 to 447 (162 residues), 83.4 bits, see alignment E=4e-27 PF00271: Helicase_C" amino acids 494 to 598 (105 residues), 67 bits, see alignment E=4.3e-22 PF19833: RecG_dom3_C" amino acids 627 to 684 (58 residues), 49.1 bits, see alignment 1.2e-16

Best Hits

Swiss-Prot: 58% identical to RECG_ECOLI: ATP-dependent DNA helicase RecG (recG) from Escherichia coli (strain K12)

KEGG orthology group: K03655, ATP-dependent DNA helicase RecG [EC: 3.6.4.12] (inferred from 67% identity to psu:Psesu_0693)

Predicted SEED Role

"ATP-dependent DNA helicase RecG (EC 3.6.1.-)" in subsystem DNA-replication (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.12

Use Curated BLAST to search for 3.6.1.- or 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (704 amino acids)

>LRK54_RS11805 ATP-dependent DNA helicase RecG (Rhodanobacter denitrificans FW104-10B01)
MATRPVRPAPAIGTDPGITSVGALPGVGPALVASLARLGLERVQDLWFHLPLRYEDKTRI
STIADLRAGDRAQVEGVVEAVERGFRYRPQLKVAIGDASGQTLLLRFFHFNRAQAEQLLP
GTRLLCYGEVRHAAQGLEMVHPNYRRLDGATVAAVDESLSPVYPTTEGLGPKRLAGVIGK
ALALLPPAAQLELIPPELCAKHGLTSLRDALLYVHRPPPDAHLGQLMLGRHPAQQRLAFE
ELLTQHLSLKRMRAAVRRRRAPELGGTGELRRRLLQGLPFRLTAAQQRVGEEVARDLVRP
QPMLRLVQGDVGSGKTVVAALAALAAVESGHQVALMAPTELLAEQHLHNFRHWLQPLGIE
VEWLAGKVTGKARKQVLARVAAGAPVVVGTHALMQEGVAFARLGLVIVDEQHRFGVQQRL
ALRDKGRDGERVPHQLVLTATPIPRTLAMSAYADLDVSSIDELPPGRTPVQTVAISNARR
SEVIGRIHAACGEGRQAYWVCTLIEESEQLRAQAAEVAHVELSAALAGFRVGLIHGRMKP
KEKQAVMDAFKAGALAVLVATTVIEVGVDVPNASLMVIENSERLGLAQLHQLRGRVGRGA
VASNCVLLYQPPLGQLARERLQVMRDTNDGFRIAEKDLELRGPGEVLGTRQTGQLSFRIA
DLARDAHLLPAVQQVGEHMLAEHSQQTGQLIERWIGGAARYAHA